Format

Send to

Choose Destination
Exp Ther Med. 2018 Aug;16(2):867-873. doi: 10.3892/etm.2018.6258. Epub 2018 Jun 7.

Naringenin inhibits NG-nitro-L-arginine methyl ester-induced hypertensive left ventricular hypertrophy by decreasing angiotensin-converting enzyme 1 expression.

Author information

1
Department of Cardiology, China-Japan Friendship Hospital, Beijing 100029, P.R. China.
2
Biomedical Experimental Research, Institute of Clinical Medicine, China-Japan Friendship Hospital, Beijing 100029, P.R. China.
3
Central Laboratory of Cardiovascular Disease, China-Japan Friendship Hospital, Beijing 100029, P.R. China.
4
Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Peking University, Beijing 100191, P.R. China.
5
Key Laboratory of Molecular Cardiovascular Science, Ministry of Education, Beijing 100191, P.R. China.

Abstract

Naringenin (NGN) is a natural flavonoid that exerts antiinflammatory, antioxidant and cardioprotective effects. The present study investigated the effects of NGN on left ventricular hypertrophy in rats with NG-nitro-L-arginine methyl ester (L-NAME)-induced hypertension, and sought to determine the underlying mechanism of action. The rats received the following by gavage daily for 56 days: L-NAME (50 mg/kg/day) + NGN (100 mg/kg/day), L-NAME (50 mg/kg/day) + saline, or saline + saline. Blood pressure, heart rate and body weight were recorded. Left ventricular hypertrophy was assessed by echocardiography and hematoxylin-eosin staining. Angiotensin II (Ang II) and angiotensin-converting enzyme 1 (ACE1), which serve a pivotal role in cardiac remodeling, were evaluated by ELISA, reverse transcription-quantitative polymerase chain reaction and western blot analysis. NGN had no significant effect on body weight, heart rate or blood pressure. The extent of left ventricular hypertrophy in the L-NAME + NGN group was lower than in the L-NAME + saline group on day 56. NGN decreased Ang II and ACE1 protein levels in myocardial tissues. In conclusion, Ang II and ACE1 expression in cardiac tissue was inhibited by NGN in L-NAME-treated rats, which may contribute to the inhibitory effects of NGN on left ventricular hypertrophy that is induced by pressure overload.

KEYWORDS:

NG-nitro-L-arginine methyl ester; angiotensin II; angiotensin-converting enzyme 1; left ventricular hypertrophy; naringenin

Supplemental Content

Full text links

Icon for PubMed Central
Loading ...
Support Center