Format

Send to

Choose Destination
Mucosal Immunol. 2018 Nov;11(6):1684-1693. doi: 10.1038/s41385-018-0047-y. Epub 2018 Aug 15.

High-dimensional immune phenotyping and transcriptional analyses reveal robust recovery of viable human immune and epithelial cells from frozen gastrointestinal tissue.

Author information

1
Division of Gastroenterology, Hepatology and Nutrition, Boston Children's Hospital, Boston, MA, 02115, USA.
2
Department of Pediatrics and Newborn Medicine, Brigham and Women's Hospital, Boston, MA, 02115, USA.
3
Harvard Medical School, Boston, MA, 02115, USA.
4
Icahn School of Medicine at Mount Sinai, New York, NY, 10029, USA.
5
Division of Gastroenterology, Brigham and Women's Hospital, Boston, MA, 02115, USA.
6
Benaroya Research Institute, Seattle, WA, 98101, USA.
7
Department of Medicine, Division of Gastroenterology, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, 19104, USA.
8
Pfizer, Cambridge, MA, 02139, USA.
9
Department of Pathology, Boston Children's Hospital, Boston, MA, 02115, USA.
10
Division of Endocrinology, Boston Children's Hospital, Boston, MA, 02115, USA.
11
Harvard Stem Cell Institute, Cambridge, MA, 02138, USA.
12
Division of Gastroenterology, Hepatology and Nutrition, Boston Children's Hospital, Boston, MA, 02115, USA. scott.snapper@childrens.harvard.edu.
13
Harvard Medical School, Boston, MA, 02115, USA. scott.snapper@childrens.harvard.edu.
14
Division of Gastroenterology, Brigham and Women's Hospital, Boston, MA, 02115, USA. scott.snapper@childrens.harvard.edu.

Abstract

Simultaneous analyses of peripheral and mucosal immune compartments can yield insight into the pathogenesis of mucosal-associated diseases. Although methods to preserve peripheral immune cells are well established, studies involving mucosal immune cells have been hampered by lack of simple storage techniques. We provide a cryopreservation protocol allowing for storage of gastrointestinal (GI) tissue with preservation of viability and functionality of both immune and epithelial cells. These methods will facilitate translational studies allowing for batch analysis of mucosal tissue to investigate disease pathogenesis, biomarker discovery and treatment responsiveness.

PMID:
30111863
PMCID:
PMC6512331
DOI:
10.1038/s41385-018-0047-y
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Nature Publishing Group Icon for PubMed Central
Loading ...
Support Center