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PLoS One. 2018 Aug 15;13(8):e0200759. doi: 10.1371/journal.pone.0200759. eCollection 2018.

Optimization of fluorophores for chemical tagging and immunohistochemistry of Drosophila neurons.

Author information

1
Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, United States of America.
2
Division of Neurobiology, Medical Research Council Laboratory of Molecular Biology, Cambridge, United Kingdom.

Abstract

The use of genetically encoded 'self-labeling tags' with chemical fluorophore ligands enables rapid labeling of specific cells in neural tissue. To improve the chemical tagging of neurons, we synthesized and evaluated new fluorophore ligands based on Cy, Janelia Fluor, Alexa Fluor, and ATTO dyes and tested these with recently improved Drosophila melanogaster transgenes. We found that tissue clearing and mounting in DPX substantially improves signal quality when combined with specific non-cyanine fluorophores. We compared and combined this labeling technique with standard immunohistochemistry in the Drosophila brain.

PMID:
30110347
PMCID:
PMC6093644
DOI:
10.1371/journal.pone.0200759
[Indexed for MEDLINE]
Free PMC Article

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