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Acta Pharm Sin B. 2018 Jul;8(4):639-644. doi: 10.1016/j.apsb.2017.12.009. Epub 2018 Feb 1.

Limonoids from seeds of Azadirachta indica A. Juss. and their cytotoxic activity.

Author information

1
Biotechnological Institute of Chinese Materia Medica, Jinan University, Guangzhou 510632, China.
2
School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, China.
3
State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou 510642, China.

Abstract

Four new limonoid-type nortriterpenoids, 1-detigloyl-1-O-methacryloylsalannin (1), 28-deoxo-2,3-dihydronimbolide (2), 12-acetoxy-3-O-acetyl-7-O-tigloylvilasinin (3) and 12-acetoxy-3-O-acetyl-7-O-methacryloylvilasinin (4), along with five known ones, were isolated from seeds of Azadirachta indica A. Juss. Their structures were elucidated by various spectroscopic methods, including UV, IR, MS, NMR, X-ray crystallography, quantum chemical calculation, as well as by comparison of their spectroscopic data with those reported. In the in vitro cytotoxic assay, 2 showed inhibitory activity against human breast cancer MDA-MB-231 cell line with IC50 value of 7.68±1.74 μmol/L, and 5 inhibited growth of human cervical cancer Hela cell line, melanoma A375 cell line and promyelocytic leukemia HL-60 cell line, with IC50 12.00±2.08, 17.44±2.11, and 13.95±5.74 μmol/L, respectively.

KEYWORDS:

Azadirachta indica; Cytotoxic activity; Limonoid; Nortriterpenoid; Spectroscopy

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