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Genes Dev. 2018 Sep 1;32(17-18):1252-1265. doi: 10.1101/gad.312173.118. Epub 2018 Aug 14.

Distinct patterns of histone acetyltransferase and Mediator deployment at yeast protein-coding genes.

Author information

1
Department of Molecular Biology, Institute of Genetics and Genomics in Geneva, 1211 Geneva 4, Switzerland.
2
Basic Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA.
3
Department of Biology, Indiana University, Bloomington, Indiana 47405, USA.

Abstract

The transcriptional coactivators Mediator and two histone acetyltransferase (HAT) complexes, NuA4 and SAGA, play global roles in transcriptional activation. Here we explore the relative contributions of these factors to RNA polymerase II association at specific genes and gene classes by rapid nuclear depletion of key complex subunits. We show that the NuA4 HAT Esa1 differentially affects certain groups of genes, whereas the SAGA HAT Gcn5 has a weaker but more uniform effect. Relative dependence on Esa1 and Tra1, a shared component of NuA4 and SAGA, distinguishes two large groups of coregulated growth-promoting genes. In contrast, we show that the activity of Mediator is particularly important at a separate, small set of highly transcribed TATA-box-containing genes. Our analysis indicates that at least three distinct combinations of coactivator deployment are used to generate moderate or high transcription levels and suggests that each may be associated with distinct forms of regulation.

KEYWORDS:

Esa1; Gcn5; Mediator; Tra1; histone acetylation; transcription

PMID:
30108132
PMCID:
PMC6120713
[Available on 2019-03-01]
DOI:
10.1101/gad.312173.118
[Indexed for MEDLINE]

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