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Genome Biol. 2018 Aug 14;19(1):113. doi: 10.1186/s13059-018-1495-0.

QsRNA-seq: a method for high-throughput profiling and quantifying small RNAs.

Author information

1
Faculty of Biology, Technion - Israel Institute of Technology, Technion City, 32000, Haifa, Israel.
2
Faculty of Biology, Technion - Israel Institute of Technology, Technion City, 32000, Haifa, Israel. ayeletla@technion.ac.il.

Abstract

The ability to profile and quantify small non-coding RNAs (sRNAs), specifically microRNAs (miRNAs), using high-throughput sequencing is challenging because of their small size. We developed QsRNA-seq, a method for preparation of sRNA libraries for high-throughput sequencing that overcomes this difficulty by enabling a gel-free separation of fragments shorter than 100 nt that differ only by 20 nt in length. The method allows the use of unique molecular identifiers for quantification and is more amenable to automation than gel-based methods. We show that QsRNA-seq gives very accurate, comprehensive, and reproducible results by looking at miRNAs in Caenorhabditis elegans embryos and larvae.

KEYWORDS:

C. elegans; High-throughput sequencing; miRNA

PMID:
30107842
PMCID:
PMC6090667
DOI:
10.1186/s13059-018-1495-0
[Indexed for MEDLINE]
Free PMC Article

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