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Br J Nutr. 2018 Oct;120(7):751-762. doi: 10.1017/S0007114518001824. Epub 2018 Aug 14.

7-Hydroxymatairesinol improves body weight, fat and sugar metabolism in C57BJ/6 mice on a high-fat diet.

Author information

1
1Clinical Chemistry Laboratory,Diagnostic Department,ASST Spedali Civili di Brescia,P. Le Spedali Civili 1, 25123 Brescia,Italy.
2
3Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna (IZSLER),"Bruno Ubertini", Via Bianchi, 9, 25124 Brescia,Italy.
3
4Human Pathology,School of Medicine,University of Brescia,P. Le Spedali Civili 1, 25123 Brescia,Italy.
4
2Department of Molecular and Translational Medicine,University of Brescia,Via Valsabbina 1, 25123 Brescia,Italy.
5
5Linnea SA,via Cantonale 123,CH-6595 Riazzino,Switzerland.
6
6Laboratory of Industrial Toxicology,Department of Medicine and Surgery,University of Parma,43126 Parma,Italy.
7
7Department of Medical and Surgical Specialties,Radiological Sciences and Public Health,Section of Public Health and Human Sciences,University of Brescia,P. Le Spedali Civili 1, 25123 Brescia,Italy.
8
8Laboratory of Wood and Paper Chemistry,Åbo Akademi University,20500 Turku,Finland.

Abstract

7-Hydroxymatairesinol (7-HMR) is a plant lignan abundant in various concentrations in plant foods. The objective of this study was to test HMRLignan™, a purified form of 7-HMR, and the corresponding Picea abies extract (total extract P. abies; TEP) as dietary supplements on a background of a high-fat diet (HFD)-induced metabolic syndrome in mice and in the 3T3-L1 adipogenesis model. Mice, 3 weeks old, were fed a HFD for 60 d. Subgroups were treated with 3 mg/kg body weight 7-HMR (HMRLignan™) or 10 mg/kg body weight TEP by oral administration. 7-HMR and TEP limited the increase in body weight (-11 and -13 %) and fat mass (-11 and -18 %) in the HFD-fed mice. Epididymal adipocytes were 19 and -12 % smaller and the liver was less steatotic (-62 and -65 %). Serum lipids decreased in TEP-treated mice (-11 % cholesterol, -23 % LDL and -15 % TAG) and sugar metabolism was ameliorated by both lignan preparations, as shown by a more than 70 % decrease in insulin secretion and insulin resistance. The expression of several metabolic genes was modulated by the HFD with an effect that was reversed by lignan. In 3T3-L1 cells, the 7-HMR metabolites enterolactone (ENL) and enterodiol (END) showed a 40 % inhibition of cell differentiation accompanied by the inhibited expression of the adipogenic genes PPARγ, C/EBPα and aP2. Furthermore, END and ENL caused a 10 % reduction in TAG uptake in HEPA 1-6 hepatoma cells. In conclusion, 7-HMR and TEP reduce metabolic imbalances typical of the metabolic syndrome and obesity in male mice, whereas their metabolites inhibit adipogenesis and lipid uptake in vitro.

KEYWORDS:

7-HMR 7-hydroxymatairesinol; DMSO dimethylsulfoxide; END enterodiol; ENL enterolactone; HFD high-fat diet; LFD low-fat diet; PA palmitic acid; TEP total Picea abies extract; TFEB transcription factor EB; 7-Hydroxymatairesinol; Adipocyte differentiation; Adipose deposition; Lignan; Metabolism; Obesity

PMID:
30105962
DOI:
10.1017/S0007114518001824

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