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Biochimie. 2018 Aug 10. pii: S0300-9084(18)30241-4. doi: 10.1016/j.biochi.2018.08.004. [Epub ahead of print]

A chronic LPS-induced low-grade inflammation fails to reproduce in lean mice the impairment of preference for oily solution found in diet-induced obese mice.

Author information

1
UMR 1231, Lipides/Nutrition/Cancer, INSERM/AgroSupDijon/Univ, Bourgogne-Franche Comté, F21000, Dijon, France; Physiologie de la Nutrition & Toxicologie (NUTox team), F21000, Dijon, France; LipSTIC LabEx, Fondation de Coopération Scientifique Bourgogne-Franche Comté, F21000, Dijon, France. Electronic address: arnaud.bernard@u-bourgogne.fr.
2
UMR 1231, Lipides/Nutrition/Cancer, INSERM/AgroSupDijon/Univ, Bourgogne-Franche Comté, F21000, Dijon, France; Physiologie de la Nutrition & Toxicologie (NUTox team), F21000, Dijon, France; LipSTIC LabEx, Fondation de Coopération Scientifique Bourgogne-Franche Comté, F21000, Dijon, France.
3
UMR 1231, Lipides/Nutrition/Cancer, INSERM/AgroSupDijon/Univ, Bourgogne-Franche Comté, F21000, Dijon, France; LipSTIC LabEx, Fondation de Coopération Scientifique Bourgogne-Franche Comté, F21000, Dijon, France; Team Pathophysiology of Dyslipidemia, F21000, Dijon, France.
4
UMR1260, INRA/1062, INSERM/Univ, Aix-Marseille, F13000, Marseille, France.
5
UMR 1231, Lipides/Nutrition/Cancer, INSERM/AgroSupDijon/Univ, Bourgogne-Franche Comté, F21000, Dijon, France; Lipness team, F21000, Dijon, France; LipSTIC LabEx, Fondation de Coopération Scientifique Bourgogne-Franche Comté, F21000, Dijon, France.
6
UMR 1231, Lipides/Nutrition/Cancer, INSERM/AgroSupDijon/Univ, Bourgogne-Franche Comté, F21000, Dijon, France; Physiologie de la Nutrition & Toxicologie (NUTox team), F21000, Dijon, France; LipSTIC LabEx, Fondation de Coopération Scientifique Bourgogne-Franche Comté, F21000, Dijon, France. Electronic address: pbesnard@u-bourgogne.fr.

Abstract

Diet-induced obesity (DIO) is associated with a decreased oral fat detection in rodents. This alteration has been explained by an impairment of the lipid-mediated signaling in taste bud cells (TBC). However, factors responsible for this defect remain elusive. Diet rich in saturated fatty acids is known to elicit a metabolic inflammation by promoting intestinal permeation to lipopolysaccharides (LPS), Gram-negative bacteria-derived endotoxins. To determine whether a local inflammation of the gustatory tissue might explain the obese-induced impairment of the oro-sensory detection of lipids, mice were subjected to a DIO protocol. Using a combination of behavioral tests, transcriptomic analyses of gustatory papillae and biochemical assays, we have found that i) DIO elicits a pro-inflammatory genic profile in the circumvallate papillae (CVP), known to house the highest density of lingual taste buds, ii) NFkB, a key player of inflammatory process, might play a role in this transcriptomic pattern, iii) plasma LPS levels are negatively correlated with the preference for oily solution, and iv) a chronic infusion of LPS at a level similar to that found in DIO mice is not sufficient to alter the spontaneous preference for fat in lean mice. Taken together these data bring the demonstration that a saturated high fat diet elicits an inflammatory response at the level of peripheral gustatory pathway and a LPS-induced low-grade endotoxemia alone does not explain the change in the preference for dietary lipids observed in DIO mice.

KEYWORDS:

Diet-induced obesity; Food choice; Inflammation; Lipids; Lipopolysaccharides

PMID:
30102932
DOI:
10.1016/j.biochi.2018.08.004
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