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AIDS. 2018 Oct 23;32(16):2429-2431. doi: 10.1097/QAD.0000000000001976.

Accurate quantification of CXCR4-using HIV-1 variants by Illumina deep-sequencing.

Author information

1
INSERM U1043 - CNRS UMR 5282, CPTP.
2
Laboratoire de Virologie, CHU Toulouse Purpan, Toulouse.
3
Virologie, Hôpital Saint-Louis, Assistance Publique Hôpitaux de Paris, Paris.
4
INSERM UMR 941, Université de Paris Diderot, Sorbonne Paris Cité.
5
INSERM, Sorbonne Université, Institut Pierre Louis d'Épidémiologie et de Santé Publique (IPLESP), Paris, France.

Abstract

: Next-generation sequencing is a sensitive method for determining HIV-1 tropism but there is a lack of data on the quantification of X4 variants. We evaluated MiSeq and 454 GS-Junior platforms for determining HIV-1 tropism and for quantifying X4 variants. Both platforms were 93% concordant for determining HIV-1 tropism and correlated well for determining the proportion of X4 variants (Spearman correlation, ρ = 0.748; P < 0.0001). MiSeq Illumina sequencing seems to be well adapted for characterizing X4-containing samples.

PMID:
30102658
DOI:
10.1097/QAD.0000000000001976
[Indexed for MEDLINE]

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