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Leg Med (Tokyo). 2018 Sep;34:17-20. doi: 10.1016/j.legalmed.2018.08.001. Epub 2018 Aug 4.

Discriminating power of rapidly mutating Y-STRs in deep rooted endogamous pedigrees from Sindhi population of Pakistan.

Author information

1
Department of Forensic Sciences, University of Health Sciences, Lahore, Pakistan.
2
Department of Forensic Medicine, Yonsei University College of Medicine, Seoul, Republic of Korea.
3
Forensic DNA Laboratory, Center for Applied Molecular Biology, University of Punjab, Lahore, Pakistan.
4
Molecular Biology and Genetics Department, Medical Research Center, Liaquat University of Medical and Health Sciences, Jamshoro, Pakistan.
5
Department of Forensic Genetics, School of Forensic Medicine, China Medical University, Shenyang, China. Electronic address: mirzaatifadnan@gmail.com.

Abstract

Rapidly mutating Y-STRs (RM Y-STRs) have been paid much attention in recent years. The 13 RM Y-STRs (DYF387S1, DYF399S1, DYF403S1a/b, DYF404S1, DYS449, DYS518, DYS526I/II, DYS547, DYS570, DYS576, DYS612, DYS626, and DYS627) have been proved to have substantially higher haplotype diversity and discrimination capacity than conventionally used Y-STRs indicating the considerable power in paternal lineage differentiation in endogamous populations, separation of which is usually impossible with standard Y-STRs. In current study, we analyzed the RM Y-STRs and PowerPlex® Y23 System in 216 male relatives from 18 deep rooted endogamous Sindhi families from Pakistan. Mutations were frequently observed at DYF399S1, DYS449, DYS518DYS547 and DYF403S1b2 loci, which are known to mutate more rapidly than other RM Y-STRs. Overall differentiation rate with RM Y-STRs was as high as 32.88%, while those with PowerPlex® Y23 System and AmpFℓSTR® Yfiler™ kit were 6.85% and 3.65% respectively. The differentiation rate of RM Y-STRs was 29.22% and 26.03% higher than those of AmpFlSTR® Yfiler™ kit and PowerPlex® Y23 System, respectively.

KEYWORDS:

AmpFℓSTR® Yfiler™ kit; Endogamous; Mutation rate; PowerPlex® Y23 System; RM Y-STR; Sindh Pakistan

PMID:
30098581
DOI:
10.1016/j.legalmed.2018.08.001
[Indexed for MEDLINE]

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