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J Biosci Bioeng. 2019 Jan;127(1):59-65. doi: 10.1016/j.jbiosc.2018.06.015. Epub 2018 Aug 8.

Comparison of biofunctional activity of Asparagus cochinchinensis (Lour.) Merr. Extract before and after fermentation with Aspergillus oryzae.

Author information

1
Research Center of Natural Cosmeceuticals Engineering, Xiamen Medical College, No. 1999, Guankou Middle Rd., Jimei Dist., Xiamen City 361023, China. Electronic address: wanggh@livemail.tw.
2
Department of Biological Science and Technology, China University of Science and Technology, No. 245, Sec. 3, Academia Rd., Nangang Dist., Taipei City 11581, Taiwan. Electronic address: stonewave@pchome.com.tw.
3
Department of Biological Science and Technology, China University of Science and Technology, No. 245, Sec. 3, Academia Rd., Nangang Dist., Taipei City 11581, Taiwan. Electronic address: jtk0901@cc.cust.edu.tw.
4
Department of Biological Science and Technology, China University of Science and Technology, No. 245, Sec. 3, Academia Rd., Nangang Dist., Taipei City 11581, Taiwan. Electronic address: happy_each_day@kimo.com.
5
Department of Biological Science and Technology, China University of Science and Technology, No. 245, Sec. 3, Academia Rd., Nangang Dist., Taipei City 11581, Taiwan. Electronic address: HBO223080284@gmail.com.
6
Department of Biological Science and Technology, China University of Science and Technology, No. 245, Sec. 3, Academia Rd., Nangang Dist., Taipei City 11581, Taiwan. Electronic address: mickey-2068@yahoo.com.tw.
7
Department of Biological Science and Technology, China University of Science and Technology, No. 245, Sec. 3, Academia Rd., Nangang Dist., Taipei City 11581, Taiwan. Electronic address: cycheng@cc.cust.edu.tw.
8
Department of Biological Science and Technology, China University of Science and Technology, No. 245, Sec. 3, Academia Rd., Nangang Dist., Taipei City 11581, Taiwan. Electronic address: ycchung@cc.cust.edu.tw.

Abstract

Asparagus cochinchinensis root (ACR) is used in traditional Chinese medicine. In this study, ACR was first extracted with 25% ethyl acetate (EA) and then fermented by Aspergillus oryzae to enhance its antioxidant activity and evaluate its potential antityrosinase activity. The physiological activity and cytotoxicity of A. oryzae-fermented ACR extract, along with its antityrosinase activity and effects on melanogenic factor levels in human epidermal melanocytes (HEMs), were analyzed and compared with those of the unfermented extract. The results showed that the physiological activity of the fermented extract in vitro or in cells was significantly higher than that of the unfermented extract. The IC50 values for 2,2-diphenyl-1-picrylhydrazine radical scavenging activity, reducing power, and antityrosinase activity in vitro for the fermented extract were 250.6 ± 32.5, 25.7 ± 3.5, and 50.6 ± 3.1 mg/L, respectively. The fermented extract favored cellular antityrosinase activity with low melanin production in human melanoma cells compared with the unfermented extract. The inhibitory mechanism of melanin synthesis by unfermented extract was independent of the tested melanogenesis-related proteins. However, the inhibitory mechanism of the fermented extract was possibly caused by synergistic inhibition of these proteins. Thus, A. oryzae-fermented ACR extract may be used for developing new health food or cosmetic ingredients.

KEYWORDS:

Antioxidant; Cytotoxicity; Fermentation; Melanogenesis; Tyrosinase

PMID:
30097404
DOI:
10.1016/j.jbiosc.2018.06.015
[Indexed for MEDLINE]

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