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Cell. 2018 Aug 9;174(4):999-1014.e22. doi: 10.1016/j.cell.2018.06.021.

Molecular Architecture of the Mouse Nervous System.

Author information

1
Division of Molecular Neurobiology, Department of Medical Biochemistry and Biophysics, Karolinska Institute, S-17177 Stockholm, Sweden.
2
UCL Institute of Neurology, London WC1N 3BG, UK.
3
Division of Molecular Neurobiology, Department of Medical Biochemistry and Biophysics, Karolinska Institute, S-17177 Stockholm, Sweden. Electronic address: sten.linnarsson@ki.se.

Abstract

The mammalian nervous system executes complex behaviors controlled by specialized, precisely positioned, and interacting cell types. Here, we used RNA sequencing of half a million single cells to create a detailed census of cell types in the mouse nervous system. We mapped cell types spatially and derived a hierarchical, data-driven taxonomy. Neurons were the most diverse and were grouped by developmental anatomical units and by the expression of neurotransmitters and neuropeptides. Neuronal diversity was driven by genes encoding cell identity, synaptic connectivity, neurotransmission, and membrane conductance. We discovered seven distinct, regionally restricted astrocyte types that obeyed developmental boundaries and correlated with the spatial distribution of key glutamate and glycine neurotransmitters. In contrast, oligodendrocytes showed a loss of regional identity followed by a secondary diversification. The resource presented here lays a solid foundation for understanding the molecular architecture of the mammalian nervous system and enables genetic manipulation of specific cell types.

KEYWORDS:

RNA sequencing; cell type; classification; single-cell transcriptomics; transcriptomics

PMID:
30096314
PMCID:
PMC6086934
DOI:
10.1016/j.cell.2018.06.021
[Indexed for MEDLINE]
Free PMC Article

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