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Protein Sci. 2018 Sep;27(9):1680-1691. doi: 10.1002/pro.3447. Epub 2018 Aug 10.

Characterization of the two conformations adopted by the T3SS inner-membrane protein PrgK.

Author information

1
Department of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, BC V6T 1Z3, Canada.
2
Centre for Blood Research, University of British Columbia, Vancouver, BC V6T 1Z3, Canada.
3
Michael Smith Laboratories, University of British Columbia, Vancouver, BC V6T 1Z4, Canada.
4
Department of Chemistry, University of British Columbia, Vancouver, BC V6T 1Z1, Canada.

Abstract

The pathogenic bacterium Salmonella enterica serovar Typhimurium utilizes two type III secretion systems (T3SS) to inject effector proteins into target cells upon infection. The T3SS secretion apparatus (the injectisome) is a large macromolecular assembly composed of over twenty proteins, many in highly oligomeric states. A sub-structure of the injectisome, termed the basal body, spans both membranes and the periplasmic space of the bacterium. It is primarily composed of three integral membranes proteins, InvG, PrgH, and PrgK, that form ring structures through which components are secreted. In particular, PrgK possesses a periplasmic region consisting of two globular domains joined by a linker polypeptide. We showed previously that in isolation, this region adopts two distinct conformations, of with only one is observed in the assembled basal body complex. Here, using NMR spectroscopy, we further characterize these two conformations. In particular, we demonstrate that the interaction of the linker region with the first globular domain, as found in the intact basal body, is dependent upon the cis conformation of the Leu77-Pro78 peptide. Furthermore, this interaction is pH-dependent due to coupling with hydrogen bond formation between Tyr75 and His42 in its neutral Nδ1 H tautomeric form. This pH-dependent interaction may play a role in the regulation of the secretion apparatus disassembly in the context of bacterial infection.

KEYWORDS:

NMR; Salmonella enterica serovar Typhimurium; bacterial secretion systems; protein dynamics

PMID:
30095200
PMCID:
PMC6194235
[Available on 2019-09-01]
DOI:
10.1002/pro.3447

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