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Carbohydr Polym. 2018 Oct 15;198:452-461. doi: 10.1016/j.carbpol.2018.06.077. Epub 2018 Jun 18.

Structural characterization and antidiabetic potential of a novel heteropolysaccharide from Grifola frondosa via IRS1/PI3K-JNK signaling pathways.

Author information

1
College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
2
Department of Pharmacy, State University of Bangladesh, Dhaka 1205, Bangladesh.
3
College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.
4
China National Engineering Research Center of JUNCAO Technology, Fujian 350002, China.
5
College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China; China National Engineering Research Center of JUNCAO Technology, Fujian 350002, China. Electronic address: xucong1154@qq.com.
6
College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China; Department of Chemistry, University of California, Davis 95616, USA. Electronic address: zhchao@live.cn.

Abstract

A novel heteropolysaccharide from Grifola frondosa named GFP-W has been isolated and purified by DEAE Sephadex A-52 chromatography. Gas chromatography, fourier transform infrared spectroscopy, one-dimensional (1H- and 13C-) and two-dimensional (1H-1H COSY, 1H-13C HSQC, and 1H-13C HMBC) nuclear magnetic resonance spectroscopy were used to characterize its structure. The average molecular weight of GFP-W was 66.1 kDa. GFP-W mainly contained four kinds of linkage type units as β-D-GlcpA→, 1,2,6-α-Gal, →2)-α-Manp→, and →3)-α-L-Fucp-(1→. It could significantly increase the uptake of glucose in dexamethasone induced insulin resistant HepG2 cells by improving the mRNA and protein expression of insulin receptor substrate 1, phosphatidylinositol-3-kinase, and glucose transporter 4 upregulation and c-Jun N-terminal Kinase 1 downregulation. Moreover, antidiabetic activities of GFP-W were associated with significant changes in the extent of protein lysine acetylation, crotonylation, and succinylation levels. Our results provide a new hypoglycemic therapeutic role and an in-depth analysis on molecular mechanisms upon polysaccharides from G. frondosa.

KEYWORDS:

Antidiabetic; Grifola frondosa polysaccharide; Insulin signaling pathway; Protein lysine modification; Structural characterization

PMID:
30093022
DOI:
10.1016/j.carbpol.2018.06.077
[Indexed for MEDLINE]

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