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Biomaterials. 2018 Oct;181:227-239. doi: 10.1016/j.biomaterials.2018.07.056. Epub 2018 Jul 30.

In situ-forming, cell-instructive hydrogels based on glycosaminoglycans with varied sulfation patterns.

Author information

1
Leibniz Institute of Polymer Research Dresden (IPF), Max Bergmann Center of Biomaterials Dresden (MBC), Hohe Str. 6, 01069 Dresden, Germany.
2
Leibniz Institute of Polymer Research Dresden (IPF), Max Bergmann Center of Biomaterials Dresden (MBC), Hohe Str. 6, 01069 Dresden, Germany; Technische Universit├Ąt Dresden, Center for Regenerative Therapies Dresden (CRTD), Fetscherstra├če 105, 01307 Dresden, Germany.
3
Leibniz Institute of Polymer Research Dresden (IPF), Max Bergmann Center of Biomaterials Dresden (MBC), Hohe Str. 6, 01069 Dresden, Germany. Electronic address: freudenberg@ipfdd.de.

Abstract

Glycosaminoglycan (GAG)-based hydrogels were proven highly effective to direct cell fate decisions by modulating the administration of cytokines. The sulfation pattern of the GAG component critically controls its affinity to proteins and thus governs the release of cytokines from GAG-containing gel systems. To apply this principle in the design of in situ assembling materials suitable for cell embedding and injection into tissues, we developed a platform of bio-orthogonally crosslinked star-shaped poly(ethylene glycol) (starPEG)-GAG hydrogels that display variable GAG sulfation patterns. Combining rational design for tuning the hydrogel network properties and a reaction-diffusion model for predicting transport processes within the matrices, we exemplarily applied the resulting materials for tailoring morphogenic and chemotactic gradients of platelet-derived growth factor-BB (PDGF-BB) in 3D. Conditions identified with this approach were demonstrated to effectively control the fate and morphogenesis of embedded mesenchymal stem cells (MSCs). Adjusting the sulfation patterns of glycosamnioglycans used in the preparation of in situ forming hydrogels is thus concluded to create new powerful options for modulating biomolecular signals in cell fate control, paving the way for advanced 3D cultures and precision tissue engineering.

KEYWORDS:

Desulfated heparin; Glycosaminoglycans (GAG); Growth factor gradient; Hydrogels; Mesenchymal stem cells (MSC); Platelet-derived growth factor B (PDGF-BB)

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