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N Biotechnol. 2018 Oct 25;45:1-8. doi: 10.1016/j.nbt.2018.08.002. Epub 2018 Aug 4.

Antibody validation: a view from the mountains.

Author information

1
Cambridge Protein Arrays Ltd., Babraham Research Campus, Cambridge, CB22 3AT, UK. Electronic address: mike.taussig@cambridgeproteinarrays.com.
2
Cambridge Protein Arrays Ltd., Babraham Research Campus, Cambridge, CB22 3AT, UK. Electronic address: claudia@cambridgeproteinarrays.com.
3
Department of Neurobiology, Physiology and Behavior, University of California, Davis, CA, 95616, USA; Department of Physiology and Membrane Biology, University of California Davis School of Medicine, Davis, CA, 95616, USA. Electronic address: jtrimmer@ucdavis.edu.

Abstract

Validation of antibodies and other protein binders is a subject of pressing concern for the research community and one which is uppermost in the minds of all who use antibodies as research and diagnostic reagents. Assessing an antibody's fitness for purpose includes accurate ascertainment of its target specificity and suitability for the envisaged task. Moreover, standardised procedures are essential to guarantee sample quality in testing procedures. The problem of defining precise standards for antibody validation has engendered much debate in recent publications and meetings, but gradually a consensus is emerging. At the 8th Alpbach Affinity Proteomics workshop (March 2017), a panel of leaders in the antibody field discussed suggestions which could bring this complex but essential issue a step nearer to a resolution. 'Alpbach recommendations' for best practice include tailoring binder validation processes according to the intended applications and promoting greater transparency in publications and in the information available from commercial antibody developers/providers. A single approach will not fit all applications and end users must ensure that the reported validation holds for their specific requirements, highlighting the need for adequate training in the fundamentals of antibody characterisation and validation across the user community.

KEYWORDS:

Affinity proteomics; Alpbach workshop; Antibody characterization; Antibody validation; Antibody validation guidelines; Pre-analytics

PMID:
30086383
PMCID:
PMC6128662
[Available on 2019-10-25]
DOI:
10.1016/j.nbt.2018.08.002
[Indexed for MEDLINE]

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