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Poult Sci. 2018 Aug 6. doi: 10.3382/ps/pey353. [Epub ahead of print]

Improvement of post-thawed sperm quality in broiler breeder roosters by ellagic acid-loaded liposomes.

Author information

1
Nanobiotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, 1435116471 Iran.
2
Department of Animal Science, College of Agriculture, University of Tabriz, Tabriz, 5166616471 Iran.
3
INDEGSAL and Department of Molecular Biology (Cell Biology), Universidad de León, León, 24071 Spain.
4
Department of Microbial Biotechnology, School of Biology, College of Science, University of Tehran, Tehran, 1417466191 Iran.

Abstract

Liposomes could improve the delivery of substances to sperm. This study was conducted to investigate the effect of the antioxidant ellagic acid and ellagic acid-loaded liposomes on post-thawed sperm quality in broiler breeder roosters. Semen was diluted in Beltsville extender containing ellagic acid or ellagic acid-loaded liposomes (ellagic acid at 0 (control), 0.5, 1, and 2 mM) and cryopreserved. Sperm quality was evaluated post-thawing: motility characteristics (Computer-Assisted Semen Analysis), membrane functionality (HOS test), abnormal morphology, mitochondrial activity (Rhodamine 123), apoptotic status (Annexin V/Propidium iodide), malondialdehyde, and antioxidant activities (glutathione peroxidase (GPx), superoxide dismutase (SOD), and total antioxidant capacity (TAC)). The results showed that 1 mM ellagic acid-loaded liposomes improved total motility, membrane functionality, and viability comparing to 0.5 and 2 mM ellagic acid, 2 mM ellagic acid-loaded liposomes, and control group. Mitochondrial activity was significantly higher for 1 mM ellagic acid-loaded liposomes compared to the rest of the treatments, except 1 mM ellagic acid. Ellagic acid at 1 mM in both forms significantly increased GPx and TAC after freeze-thawing (no significant variation for SOD), and also yielded the lower proportion of apoptotic and dead cells. In conclusion, ellagic acid improved post-thawed sperm quality in broiler breeder roosters. The use of liposomes could further enhance the effects of ellagic acid.

PMID:
30085198
DOI:
10.3382/ps/pey353

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