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Sci Data. 2018 Aug 7;5:180142. doi: 10.1038/sdata.2018.142.

A multi-omic atlas of the human frontal cortex for aging and Alzheimer's disease research.

Author information

1
Center for Translational & Computational Neuroimmunology, Department of Neurology, Columbia University Medical Center, 630 West 168th street, New York, NY 10032, USA.
2
Cell Circuits Program, Broad Institute, 415 Main street, Cambridge MA 02142, USA.
3
Sage Bionetworks, 1100 Fairview Avenue N. Seattle WA 98109, USA.
4
Rush Alzehimer Disease Center, RUSH University, 600 South Paulina Street, Chicago IL 60612, USA.
5
Departments of Statistics and Medical Genetics and Centre for Molecular Medicine and Therapeutics, University of British Columbia, 950 West 28th Avenue, Vancouver, British Columbia BC V5Z 4H4, Canada.

Abstract

We initiated the systematic profiling of the dorsolateral prefrontal cortex obtained from a subset of autopsied individuals enrolled in the Religious Orders Study (ROS) or the Rush Memory and Aging Project (MAP), which are jointly designed prospective studies of aging and dementia with detailed, longitudinal cognitive phenotyping during life and a quantitative, structured neuropathologic examination after death. They include over 3,322 subjects. Here, we outline the first generation of data including genome-wide genotypes (n=2,090), whole genome sequencing (n=1,179), DNA methylation (n=740), chromatin immunoprecipitation with sequencing using an anti-Histone 3 Lysine 9 acetylation (H3K9Ac) antibody (n=712), RNA sequencing (n=638), and miRNA profile (n=702). Generation of other omic data including ATACseq, proteomic and metabolomics profiles is ongoing. Thanks to its prospective design and recruitment of older, non-demented individuals, these data can be repurposed to investigate a large number of syndromic and quantitative neuroscience phenotypes. The many subjects that are cognitively non-impaired at death also offer insights into the biology of the human brain in older non-impaired individuals.

PMID:
30084846
PMCID:
PMC6080491
DOI:
10.1038/sdata.2018.142
[Indexed for MEDLINE]
Free PMC Article

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