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Protein Pept Lett. 2018;25(8):757-766. doi: 10.2174/0929866525666180806110928.

Adjuvant Activity of Bursal Pentapeptide-(III-V) in Mice Immunized with the H9N2 Avian Influenza Vaccine.

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1
College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, Henan, China.

Abstract

BACKGROUND:

Bursal Pentapeptide (BPP)-(III-V) are peptides isolated from the Bursa of Fabricius (BF).

METHOD:

The peptides' adjuvant activities were evaluated in mice immunized with the H9N2 avian influenza vaccine.

RESULTS:

The results showed that BPP-IV did not promote humoral or cellular immune responses. BPP-III had no effect on antibody production, but increased populations of CD3+ and CD8+ T cells, CTL levels and proliferation activities of splenic T-lymphocytes. BPP-V increased the level of antibodies (HI, IgG and its subtype IgG1) and the cytokine IL-4, as well as the ratio of CD3+ T cells and its subsets CD4+ and CD8+ T cells, BPP-V also increased CTL level and promoted the proliferation activity of T- and B-lymphocyte. Furthermore, in animal challenge experiment, BPP-III and BPP-V reduced viral RNA copies and viral titers, and reduced inflammatory cell infiltration.

CONCLUSION:

In summary, BPP-IV did not have an effect on specific immune responses, therefore it could not reduce pathological damage in mice lungs; BPP-III provided resistance to the antigen through the cell-mediated immune response; BPP-V enhanced both humoral and cellular immune responses, therefore provided protections against the H9N2 Avian Influenza Virus (AIV). Altogether, BPP-III and BPP-V could be adjuvant candidates for the H9N2 avian influenza vaccine.

KEYWORDS:

Bursal pentapeptided-(III-V); adjuvants; avian influenza virus; cellular immune response; humoral immune response; immunoprotection test.

[Indexed for MEDLINE]

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