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ACS Chem Biol. 2018 Sep 21;13(9):2421-2426. doi: 10.1021/acschembio.8b00635. Epub 2018 Aug 13.

ABO Blood Group Antigen Decorated Giant Unilamellar Vesicles Exhibit Distinct Interactions with Plasmodium falciparum Infected Red Blood Cells.

Author information

1
Department of Chemistry, Chemical Biology , University of Copenhagen , Thorvaldsensvej 40 , 1871 Frederiksberg C , Denmark.
2
Department of Microbiology, Tumor and Cell Biology (MTC) , Karolinska Institutet , Box 280, Nobels väg 16 , SE-171 77 Stockholm , Sweden.
3
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences , Miklukho-Maklaya 16/10 , 117997 Moscow , Russian Federation.

Abstract

Severe malaria is considered to be the deadliest disease of this century, primarily among children in sub-Saharan Africa. It stems from infection by the virulent parasite Plasmodium falciparum. The pathogenesis of the disease is based on the rosetting phenomenon, which occurs during the life cycle of the parasite in red blood cells (RBCs) and promotes the binding of parasitized RBCs to healthy ones. The role of the ABO blood group antigens in relation to the phenomenon has previously only been investigated in clinical isolates obtained from malaria patients. Here, we aim to clarify their role using synthetic ABO-decorated giant unilamellar vesicles (GUVs), which serve as simple biomimetic models of RBC-size cell membranes. Our results suggest clearly and for the first time that the blood group A and O antigens have a direct impact on receptor-specific rosetting phenomena when compared to the B antigen, which only participates in rosetting to an insignificant degree. Thus, glycodecorated GUVs represent a practical tool for studying cell-surface interactions.

PMID:
30080386
DOI:
10.1021/acschembio.8b00635
[Indexed for MEDLINE]

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