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Chembiochem. 2018 Oct 18;19(20):2173-2177. doi: 10.1002/cbic.201800443. Epub 2018 Sep 26.

An Enzyme-Mediated Amplification Strategy Enables Detection of β-Lactamase Activity Directly in Unprocessed Clinical Samples for Phenotypic Detection of β-Lactam Resistance.

Author information

1
Department of Bioengineering, University of California, Berkeley, University Avenue, Berkeley, CA, 94720, USA.
2
Department of Public Health, University of California, Berkeley, University Avenue, Berkeley, CA, 94720, USA.

Abstract

Biochemical assays that can identify β-lactamase activity directly from patient samples have the potential to significantly improve the treatment of bacterial infections. However, current β-lactamase probes do not have the sensitivity needed to measure β-lactam resistance directly from patient samples. Here, we report the development of an instrument-free signal amplification technology, DETECT, that connects the activity of two enzymes in series to effectively amplify the activity of β-lactamase 40 000-fold, compared to the standard β-lactamase probe nitrocefin.

KEYWORDS:

amplification; antimicrobial resistance; beta-lactamases; diagnostics; point-of-care diagnosis

PMID:
30079487
DOI:
10.1002/cbic.201800443

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