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Angew Chem Int Ed Engl. 2018 Sep 3;57(36):11589-11593. doi: 10.1002/anie.201804682. Epub 2018 Aug 6.

Cross-Linked Aptamer-Lipid Micelles for Excellent Stability and Specificity in Target-Cell Recognition.

Author information

1
Center for Research at the Bio/Nano Interface, Department of Chemistry and Department of Physiology and Functional Genomics, Health Cancer Center, UF Genetics Institute and McKnight Brain Institute, University of Florida, Gainesville, FL, 32611, USA.
2
George and Josephine Butler Polymer Research Laboratory, Center for Macromolecular Science and Engineering, Department of Chemistry, University of Florida, Gainesville, FL, 32611-7200, USA.
3
Molecular Science and Biomedicine Laboratory (MBL), State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Life Sciences and College of Chemistry and Chemical Engineering, Aptamer Engineering, Center of Hunan Province, Hunan University, Changsha, Hunan, 410082, China.
4
Institute of Molecular Medicine, Renji Hospital, Shanghai Jiao Tong University School of Medicine, College of Chemistry and Chemical Engineering, Shanghai, 200240, China.

Abstract

The specific binding ability of DNA-lipid micelles (DLMs) can be increased by the introduction of an aptamer. However, supramolecular micellar structures based on self-assemblies of amphiphilic DLMs are expected to demonstrate low stability when interacting with cell membranes under certain conditions, which could lead to a reduction in selectivity for targeting cancer cells. We herein report a straightforward cross-linking strategy that relies on a methacrylamide branch to link aptamer and lipid segments. By an efficient photoinduced polymerization process, covalently linked aptamer-lipid units help stabilize the micelle structure and enhance aptamer probe stability, further improving the targeting ability of the resulting nanoassembly. Besides the development of a facile cross-linking method, this study clarifies the relationship between aptamer-lipid concentration and the corresponding binding ability.

KEYWORDS:

aptamer-lipid micelles; biostability; cross-linking; self-assembly; specific cell recognition

PMID:
30079455
PMCID:
PMC6442728
[Available on 2019-09-03]
DOI:
10.1002/anie.201804682

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