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Front Plant Sci. 2018 Jul 20;9:686. doi: 10.3389/fpls.2018.00686. eCollection 2018.

Overexpression of Phosphate Transporter Gene CmPht1;2 Facilitated Pi Uptake and Alternated the Metabolic Profiles of Chrysanthemum Under Phosphate Deficiency.

Liu C1,2, Su J1,2, Stephen GK1,2, Wang H1,2, Song A1,2, Chen F1,2, Zhu Y3, Chen S1,2, Jiang J1,2.

Author information

1
College of Horticulture, Nanjing Agricultural University, Nanjing, China.
2
Key Laboratory of Landscape Agriculture, Ministry of Agriculture, Nanjing, China.
3
College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, China.

Abstract

Low availability of phosphorus (P) in the soil is the principal limiting factor for the growth of cut chrysanthemum. Plant phosphate transporters (PTs) facilitate acquisition of inorganic phosphate (Pi) and its homeostasis within the plant. In the present study, CmPht1;2 of the Pht1 family was cloned from chrysanthemum. CmPht1;2 is composed of 12 transmembrane domains and localized to the plasma membrane. Expression of CmPht1;2 in roots was induced by Pi starvation. Chrysanthemum plants with overexpression of CmPht1;2 (Oe) showed higher Pi uptake, as compared to the wild type (WT), both under Pi-starvation and Pi-sufficient conditions, and also showed a higher root biomass compared to WT in the Pi-starvation conditions. Seven days after the P-deficiency treatment, 85 distinct analytes were identified in the roots and 27 in the shoots between the Oe1 plant and WT, in which sophorose, sorbitol (sugars), hydroxybutyric acid (organic acids), and ornithine (amino acid) of CmPht1;2 overexpressing chrysanthemum are specific responses to P-starvation.

KEYWORDS:

Chrysanthemum morifolium; CmPht1;2; metabolome; phosphate transporter; phosphorus

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