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Curr Opin Biotechnol. 2018 Dec;54:128-137. doi: 10.1016/j.copbio.2018.07.001. Epub 2018 Aug 2.

Towards better understanding of industrial cell factories: novel approaches for 13C metabolic flux analysis in complex nutrient environments.

Author information

1
Institute of Systems Biotechnology, Saarland University, Germany.
2
Institute of Systems Biotechnology, Saarland University, Germany. Electronic address: christoph.wittmann@uni-saarland.de.

Abstract

The quantification of intracellular fluxes via 13C metabolic flux analysis has become one of the major techniques to support systems-based metabolic engineering of industrial production hosts. Commonly, 13C studies are conducted using minimal media, however, a great variety of industrial processes rely on complex medium compounds. The use of parallel 13C tracer experiments as well as analytical multi-readout of labeling data together with model-based simulation offer a great starting point to tackle such complex systems. Prominent examples will highlight the recent progress made.

PMID:
30077860
DOI:
10.1016/j.copbio.2018.07.001
[Indexed for MEDLINE]

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