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Appl Microbiol Biotechnol. 2018 Oct;102(19):8319-8327. doi: 10.1007/s00253-018-9267-z. Epub 2018 Aug 3.

Metabolic engineering of Clostridium acetobutylicum for the production of butyl butyrate.

Author information

1
Institute of Agriculture & Life Science (IALS), Department of Agricultural Chemistry and Food Science Technology, Division of Applied Life Science (BK21 Plus Program), Gyeongsang National University, Jinju, Republic of Korea.
2
Department of Chemical and Biomolecular Engineering (BK21 Plus Program), BioProcess Engineering Research Center, Institute for the BioCentury, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, Republic of Korea. leesy@kaist.ac.kr.
3
Institute of Agriculture & Life Science (IALS), Department of Agricultural Chemistry and Food Science Technology, Division of Applied Life Science (BK21 Plus Program), Gyeongsang National University, Jinju, Republic of Korea. jangys@gnu.ac.kr.

Abstract

Butyl butyrate is widely used as a fragrance additive for foods and beverages. The first step in the currently used process is the production of precursors, including butanol and butyrate, from petroleum using chemical catalysts, followed by the conversion of precursors to butyl butyrate by immobilized lipase. In this work, we engineered Clostridium acetobutylicum for the selective, one-step production of butyl butyrate from glucose. C. acetobutylicum ATCC 824, possessing a strong carbon flux that yields butanol and butyryl-CoA, was selected as a host and was engineered by introducing alcohol acyltransferases (AATs) from Fragaria x ananassa (strawberry) or Malus sp. (apple). Batch culture of the engineered C. acetobutylicum strain CaSAAT expressing the strawberry SAAT gene produced 50.07 mg/L of butyl butyrate with a selectivity of 84.8% of total esters produced. Also, the engineered C. acetobutylicum strain CaAAAT expressing the apple AAAT gene produced 40.60 mg/L of butyl butyrate with a selectivity of 87.4%. This study demonstrated the feasibility of the one-step fermentation of butyl butyrate from glucose in the engineered C. acetobutylicum, as a proof of concept.

KEYWORDS:

Alcohol acyltransferase; Butanol; Butyl butyrate; Clostridium acetobutylicum; Ester

PMID:
30076425
DOI:
10.1007/s00253-018-9267-z
[Indexed for MEDLINE]

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