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J Agric Food Chem. 2018 Aug 22;66(33):8772-8782. doi: 10.1021/acs.jafc.8b02598. Epub 2018 Aug 13.

Characterization of the Genes Involved in Malic Acid Metabolism from Pear Fruit and Their Expression Profile after Postharvest 1-MCP/Ethrel Treatment.

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Centre of Pear Engineering Technology Research, State Key Laboratory of Crop Genetics and Germplasm Enhancement , Nanjing Agricultural University , Nanjing , Jiangsu 210095 , China.
USDA, ARS, U.S. Horticultural Research Laboratory , 2001 S. Rock Road , Ft. Pierce , Florida 34945 , United States.


In this study, five genes involved in malic acid (MA) metabolism, including a cytosolic NAD-dependent malate dehydrogenase gene ( cyNAD-MDH), a cytosolic NADP-dependent malic enzyme gene ( cyNADP-ME), two vacuolar H+-ATPase genes ( vVAtp1 and vVAtp2), and one vacuolar inorganic pyrophosphatase gene ( vVPp), were characterized from pear fruit based on bioinformatic and experimental analysis. Their expression profile in "Housui" pear was tissue-specific, and their expression patterns during fruit development were diverse. During "Housui" pear storage, MA content decreased, which was associated with the downregulated transcripts of MA metabolism-related genes and cyNAD-MDH activity and higher cyNADP-ME activity. The response of MA metabolism to postharvest 1.5 μL L-1 1-MCP fumigation and 0.5 mL L-1 ethrel dipping was distinct: 1-MCP fumigation upregulated gene expression and cyNAD-MDH activity and suppressed cyNADP-ME activity, and thus maintained higher MA abundance when compared with those in the control; on the other hand, an opposite behavior was observed in ethrel-treated fruit.


1-MCP; enzyme activity; ethrel; gene expression; malic acid; pear

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