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Methods Mol Biol. 2018;1832:185-203. doi: 10.1007/978-1-4939-8663-7_10.

Bioinformatic Analysis of Nucleosome and Histone Variant Positioning.

Author information

1
Neurobiology Center, Nencki Institute of Experimental Biology, Warsaw, Poland.
2
Department of Molecular Biology, Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA. tolstorukov@molbio.mgh.harvard.edu.

Abstract

Assays profiling nucleosome positioning and occupancy are often coupled with high-throughput sequencing, which results in generation of large data sets. These data sets require processing in specialized computational pipelines to yield useful information. Here, we describe main steps of such a pipeline, and discuss bioinformatic and statistical aspects of assessing data quality, as well as data visualization and further analysis.

KEYWORDS:

Data processing and analysis; Histone variants; MNase; Next generation sequencing; Nucleosome positioning

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