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Methods Mol Biol. 2018;1832:185-203. doi: 10.1007/978-1-4939-8663-7_10.

Bioinformatic Analysis of Nucleosome and Histone Variant Positioning.

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Neurobiology Center, Nencki Institute of Experimental Biology, Warsaw, Poland.
Department of Molecular Biology, Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA.


Assays profiling nucleosome positioning and occupancy are often coupled with high-throughput sequencing, which results in generation of large data sets. These data sets require processing in specialized computational pipelines to yield useful information. Here, we describe main steps of such a pipeline, and discuss bioinformatic and statistical aspects of assessing data quality, as well as data visualization and further analysis.


Data processing and analysis; Histone variants; MNase; Next generation sequencing; Nucleosome positioning

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