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Endocr Connect. 2018 Aug 2. pii: EC-18-0205. doi: 10.1530/EC-18-0205. [Epub ahead of print]

Circulating miR-148a associates with sensitivity to adiponectin levels in human metabolic surgery for weight loss.

Author information

1
M Ariza-Nieto, Research and Development, epiWELL, LLC, Ithaca, United States magnoliaariza@gmail.com.
2
J Alley, Donald Guthrie Foundation for Education and Research, Guthrie Clinic Sayre, Sayre, United States.
3
S Samy, Donald Guthrie Foundation for Education and Research, Guthrie Clinic Sayre, Sayre, United States.
4
L Fitzgerald, Donald Guthrie Foundation for Education and Research, Guthrie Clinic Sayre, Sayre, United States.
5
F Vermeylen, Cornell Statistical Consulting Unit, Cornell University, Ithaca, United States.
6
M Shuler, Meinig School of Biomedical Engineering, Cornell University, Ithaca, United States.
7
J Aleman, Endocrinology, New York University Langone Medical Center, New York, United States.

Abstract

OBJECTIVE:

We sought to discover secreted biomarkers to monitor the recovery of physiological adiponectin levels with metabolic surgery, focusing on epigenetic changes that might predict adiponectin function.

DESIGN:

We conducted a prospective observational study of patients undergoing metabolic surgery by Roux-en-Y gastric bypass (RYGB) for weight loss in a single center (IRB GHS # 1207-27).

METHODS:

All patients (n=33; 27 females; 6 males) signed informed consent. Metabolites, adiponectin, and miR-148a were measured in fasting plasma. We followed MIQE for transcript profiles.

RESULTS:

Patients lost on average 47±12% excess BMI (%EBMI) after 12 weeks. Adiponectin pre, post or delta [post minus pre] did not correlate with %EBMIL. A decrease in adiponectin following weight loss surgery was observed in a subset of patients, chi-square test of independence rejects the null hypotheses that the liver DNMT1 and delta adiponectin are independent (Chi-square statistics χ2 38 = 6.9205, P= 0.00852, n=33), as well as miR-148a and delta adiponectin are independent (Chisquare statistics χ2 40 = 9.6823, P= 0.00186, n=33). The presence of plasma miR-148a allows identification of patients that appear to be adiponectin insensitive at baseline.

CONCLUSION:

We combined the presence of plasma miR-148a, the concentration of total adiponectin and the expression of DNA methyltransferase 1 (DNMT1) in liver biopsy tissue to identify patients with non-physiological adiponectin. Weight loss and physical activity interventions complemented with the new method presented here could serve to monitor the physiological levels of adiponectin, thought to be important for long-term weight loss maintenance.

PMID:
30072446
DOI:
10.1530/EC-18-0205
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