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Mar Drugs. 2018 Aug 1;16(8). pii: E264. doi: 10.3390/md16080264.

Sargassum Fusiforme Polysaccharide SFP-F2 Activates the NF-κB Signaling Pathway via CD14/IKK and P38 Axes in RAW264.7 Cells.

Author information

1
College of Life and Environmental Science, Wenzhou University, Wenzhou 325035, China. lchen3@unbc.ca.
2
Natural Resources and Environmental Studies Program, University of Northern British Columbia, Prince George, BC V2N 4Z9, Canada. lchen3@unbc.ca.
3
College of Life and Environmental Science, Wenzhou University, Wenzhou 325035, China. chenpeichao@wzu.edu.cn.
4
College of Life and Environmental Science, Wenzhou University, Wenzhou 325035, China. 15451382280@stu.wzu.edu.cn.
5
College of Life and Environmental Science, Wenzhou University, Wenzhou 325035, China. 15451382279@stu.wzu.edu.cn.
6
College of Life and Environmental Science, Wenzhou University, Wenzhou 325035, China. 14112822281@stu.wzu.edu.cn.
7
College of Life and Environmental Science, Wenzhou University, Wenzhou 325035, China. 13112822263@stu.wzu.edu.cn.
8
College of Life and Environmental Science, Wenzhou University, Wenzhou 325035, China. yupingwzu@gmail.com.
9
College of Life and Environmental Science, Wenzhou University, Wenzhou 325035, China. wmj@wzu.edu.cn.
10
College of Life and Environmental Science, Wenzhou University, Wenzhou 325035, China. dawn_zhang_79@hotmail.com.

Abstract

Sargassum fusifrome is considered a "longevity vegetable" in Asia. Sargassum fusifrome polysaccharides exhibit numerous biological activities, specially, the modulation of immune response via the NF-κB signaling pathway. However, the precise mechanisms by which these polysaccharides modulate the immune response through the NF-κB signaling pathway have not been elucidated. In this study, we purified and characterized a novel fraction of Sargassum fusifrome polysaccharide and named it SFP-F2. SFP-F2 significantly upregulated the production of the cytokines TNF-α, IL-1β and IL-6 in RAW264.7 cells. It also activated the NF-κB signaling pathway. Data obtained from experiments carried out with specific inhibitors (PDTC, BAY 11-7082, IKK16 and SB203580) suggested that SFP-F2 activated the NF-κB signaling pathway via CD14/IKK and P38 axes. SFP-F2 could therefore potentially exert an immune-enhancement effect through inducing the CD14/IKK/NF-κB and P38/NF-κB signaling pathways.

KEYWORDS:

CD14; NF-κB signaling pathway; P38; RAW264.7 cell; Sargassum fusifrome; polysaccharides

PMID:
30071655
PMCID:
PMC6117693
DOI:
10.3390/md16080264
[Indexed for MEDLINE]
Free PMC Article

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