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Virology. 2018 Sep;522:281-291. doi: 10.1016/j.virol.2018.07.001. Epub 2018 Jul 30.

Nanovirus DNA-N encodes a protein mandatory for aphid transmission.

Author information

1
Institut des Sciences du Végétal, CNRS, 91198 Gif sur Yvette, France.
2
Im Spargelfeld 1, 38162 Cremlingen, Germany.
3
Institute for Molecular Biotechnology (Biology VII), RWTH Aachen University, Worringerweg 1, 52074 Aachen, Germany.
4
Julius Kühn Institute (JKI), Bundesforschungsinstitut für Kulturpflanzen, Institut für Epidemiologie und Pathogendiagnostik, 38104 Braunschweig, Germany.
5
Institut des Sciences du Végétal, CNRS, 91198 Gif sur Yvette, France; Institute for Integrative Biology of the Cell, UMR 9198, CNRS, Université Paris-Sud, CEA, Avenue de la Terrasse, 91198 Gif sur Yvette, France.
6
Institut des Sciences du Végétal, CNRS, 91198 Gif sur Yvette, France; Institute for Integrative Biology of the Cell, UMR 9198, CNRS, Université Paris-Sud, CEA, Avenue de la Terrasse, 91198 Gif sur Yvette, France. Electronic address: tania.timtchenko@i2bc.paris-saclay.fr.

Abstract

Nanoviruses possess a multipartite single-stranded DNA genome and are naturally transmitted to plants by various aphid species in a circulative non-propagative manner. Using the cloned genomic DNAs of faba bean necrotic stunt virus (FBNSV) for reconstituting nanovirus infections we analyzed the necessity of different virus components for infection and transmission by aphids. We found that in the absence of DNA-U1 and DNA-U2 symptom severity decreased, and in the absence of DNA-U1 the transmission efficiency decreased. Most significantly, we demonstrated that the protein encoded by DNA-N (NSP) is mandatory for aphid transmission. Moreover, we showed that the NSP of FBNSV could substitute for that of a distantly related nanovirus, pea necrotic yellow dwarf virus. Altering the FBNSV NSP by adding 13 amino acids to its carboxy-terminus resulted in an infectious but non-transmissible virus. We demonstrate that the NSP acts as a nanovirus transmission factor, the existence of which had been hypothesized earlier.

KEYWORDS:

Acyrthosiphon pisum; Aphis craccivora; Faba bean; Plant virus; Reassortment; Segmented single-stranded DNA genome; Transmission helper factor

PMID:
30071404
DOI:
10.1016/j.virol.2018.07.001
[Indexed for MEDLINE]
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