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Elife. 2018 Aug 2;7. pii: e36435. doi: 10.7554/eLife.36435.

Loss of Fam60a, a Sin3a subunit, results in embryonic lethality and is associated with aberrant methylation at a subset of gene promoters.

Author information

1
Developmental Genetics Group, Graduate School of Frontier Biosciences, Osaka University, Suita, Japan.
2
Laboratory for Organismal Patterning, RIKEN Center for Developmental Biology, Kobe, Japan.
3
Phyloinformatics Unit, RIKEN Center for Life Science Technologies, Kobe, Japan.
4
Laboratory for Phyloinformatics, RIKEN Center for Biosystems Dynamics Research, Kobe, Japan.
#
Contributed equally

Abstract

We have examined the role of Fam60a, a gene highly expressed in embryonic stem cells, in mouse development. Fam60a interacts with components of the Sin3a-Hdac transcriptional corepressor complex, and most Fam60a-/- embryos manifest hypoplasia of visceral organs and die in utero. Fam60a is recruited to the promoter regions of a subset of genes, with the expression of these genes being either up- or down-regulated in Fam60a-/- embryos. The DNA methylation level of the Fam60a target gene Adhfe1 is maintained at embryonic day (E) 7.5 but markedly reduced at E9.5 in Fam60a-/- embryos, suggesting that DNA demethylation is enhanced in the mutant. Examination of genome-wide DNA methylation identified several differentially methylated regions, which were preferentially hypomethylated, in Fam60a-/- embryos. Our data suggest that Fam60a is required for proper embryogenesis, at least in part as a result of its regulation of DNA methylation at specific gene promoters.

KEYWORDS:

DNA methylation; Sin3a; developmental biology; epigenetics; mouse; regenerative medicine; stem cell; stem cells

PMID:
30070635
PMCID:
PMC6072441
DOI:
10.7554/eLife.36435
[Indexed for MEDLINE]
Free PMC Article

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