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PeerJ. 2018 Jul 25;6:e5369. doi: 10.7717/peerj.5369. eCollection 2018.

A novel cecropin B-derived peptide with antibacterial and potential anti-inflammatory properties.

Wang J1,2, Ma K1,2, Ruan M1,2, Wang Y1,2, Li Y1,2,3, Fu YV4, Song Y5, Sun H1,2,6, Wang J1,2,7.

Author information

1
High Magnetic Field Laboratory, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, China.
2
Key Laboratory of High Magnetic Field and Ion Beam Physical Biology, Chinese Academy of Sciences, Hefei, China.
3
The First Affiliated Hospital of Xinxiang Medical University, Xinxiang, China.
4
State Key Laboratory of Microbial Resources, Institution of Microbiology, Chinese Academy of Sciences, Beijing, China.
5
School of Chemistry and Chemical Engineering, Hefei University of Technology, Hefei, China.
6
School of Food and Biological Engineering, Zhengzhou University of Light Industry, Zhengzhou, China.
7
Institute of Physical Science and information Technology, Anhui University, Hefei, China.

Abstract

Cecropins, originally found in insects, are a group of cationic antimicrobial peptides. Most cecropins have an amphipathic N-terminal segment and a largely hydrophobic C-terminal segment, and normally form a helix-hinge-helix structure. In this study, we developed the novel 32-residue cecropin-like peptide cecropin DH by deleting the hinge region (Alanine-Glycine-Proline) of cecropin B isolated from Chinese oak silk moth, Antheraea pernyi. Cecropin DH possesses effective antibacterial activity, particularly against Gram-negative bacteria, with very low cytotoxicity against mammalian cells. Interactions between cecropin DH and the highly anionic lipopolysaccharide (LPS) component of the Gram-negative bacterial outer membrane indicate that it is capable of dissociating LPS micelles and disrupting LPS aggregates into smaller assemblies, which may play a vital role in its antimicrobial activity. Using LPS-stimulated mouse macrophage RAW264.7 cells, we found that cecropin DH exerted higher potential anti-inflammatory activity than cecropin B, as demonstrated by the inhibition of pro-inflammatory cytokines nitric oxide production and secretion of tumor necrosis factor-α. In conclusion, cecropin DH has potential as a therapeutic agent for both antibacterial and anti-inflammatory applications.

KEYWORDS:

Antibacterial activity; Cecropin DH; Inhibition of pro-inflammatory cytokines; LPS small micelle formation

Conflict of interest statement

The authors declare that they have no competing interests.

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