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Molecules. 2018 Jul 31;23(8). pii: E1912. doi: 10.3390/molecules23081912.

Physicochemical Characterization, Antioxidant and Immunostimulatory Activities of Sulfated Polysaccharides Extracted from Ascophyllum nodosum.

Chen L1,2,3, Wang Y4, Yang H5, Li H6,7,8, Xu W9,10, Chen G11, Zhu H12.

Author information

1
College of Chemistry and Molecular Engineering, Nanjing Tech University, Nanjing 211816, China. chenlg@ycit.cn.
2
School of Marine and Bioengineering, Yancheng Institute of Technology, Yancheng 224051, China. chenlg@ycit.cn.
3
Jiangsu Key Laboratory of Biochemistry and Biotechnology of Marine Wetland, Yancheng Institute of Technology, Yancheng 224051, China. chenlg@ycit.cn.
4
College of Chemistry and Molecular Engineering, Nanjing Tech University, Nanjing 211816, China. 1954357344@njtech.edu.cn.
5
School of Marine and Bioengineering, Yancheng Institute of Technology, Yancheng 224051, China. yangh96@yahoo.com.
6
College of Chemistry and Molecular Engineering, Nanjing Tech University, Nanjing 211816, China. zllz@ycit.cn.
7
School of Marine and Bioengineering, Yancheng Institute of Technology, Yancheng 224051, China. zllz@ycit.cn.
8
Jiangsu Key Laboratory of Biochemistry and Biotechnology of Marine Wetland, Yancheng Institute of Technology, Yancheng 224051, China. zllz@ycit.cn.
9
School of Marine and Bioengineering, Yancheng Institute of Technology, Yancheng 224051, China. xuweiyc@163.com.
10
Jiangsu Key Laboratory of Biochemistry and Biotechnology of Marine Wetland, Yancheng Institute of Technology, Yancheng 224051, China. xuweiyc@163.com.
11
College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China. guijiechen@njau.edu.cn.
12
College of Chemistry and Molecular Engineering, Nanjing Tech University, Nanjing 211816, China. zhuhj@njtech.edu.cn.

Abstract

Polysaccharides from Ascophyllum nodosum (AnPS) were extracted and purified via an optimized protocol. The optimal extraction conditions were as follows: extraction time of 4.3 h, extraction temperature of 84 °C and ratio (v/w, mL/g) of extraction solvent (water) to raw material of 27. The resulting yield was 9.15 ± 0.23% of crude AnPS. Two fractions, named AnP1-1 and AnP2-1 with molecular weights of 165.92 KDa and 370.68 KDa, were separated from the crude AnPS by chromatography in DEAE Sepharose Fast Flow and Sephacryl S-300, respectively. AnP1-1 was composed of mannose, ribose, glucuronic acid, glucose and fucose, and AnP2-1 was composed of mannose, glucuronic acid, galactose and fucose. AnPS, AnP1-1 and AnP2-1 exhibited high scavenging activities against ABTS radical and superoxide radical, and showed protective effect on H₂O₂-induced oxidative injury in RAW264.7 cells. Furthermore, the immunostimulatory activities of AnP1-1 and AnP2-1 were evaluated by Caco-2 cells, the results showed both AnP1-1 and AnP2-1 could significantly promote the production of immune reactive molecules such as interleukin (IL)-8, IL-1β, interferon (IFN)-γ, and tumor necrosis factor (TNF)-α. Therefore, the results suggest that AnPS and its two fractions may be explored as a potential functional food supplement.

KEYWORDS:

Ascophyllum nodosum; antioxidant activity; immunostimulatory activity; polysaccharides

PMID:
30065217
PMCID:
PMC6222602
DOI:
10.3390/molecules23081912
[Indexed for MEDLINE]
Free PMC Article

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