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Cytometry A. 2018 Aug;93(8):822-828. doi: 10.1002/cyto.a.23386. Epub 2018 Jul 31.

Mechanical property characterization of hundreds of single nuclei based on microfluidic constriction channel.

Chang CC1, Wang K2,3, Zhang Y2,3, Chen D2,3, Fan B2,3, Hsieh CH4, Wang J2,3, Wu MH1,4, Chen J2,3.

Author information

1
Graduate Institute of Biochemical and Biomedical Engineering, Chang Gung University, Taoyuan City, Taiwan.
2
State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing, People's Republic of China.
3
School of Electronic, Electrical and Communication Engineering/School of Future Technology, University of Chinese Academy of Sciences, Beijing, People's Republic of China.
4
Division of Haematology/Oncology, Department of Internal Medicine, Chang Gung Memorial Hospital at Linkou, Taoyuan City, Taiwan.

Abstract

As label-free biomarkers, the mechanical properties of nuclei are widely treated as promising biomechanical markers for cell type classification and cellular status evaluation. However, previously reported mechanical parameters were derived from only around 10 nuclei, lacking statistical significances due to low sample numbers. To address this issue, nuclei were first isolated from SW620 and A549 cells, respectively, using a chemical treatment method. This was followed by aspirating them through two types of microfluidic constriction channels for mechanical property characterization. In this study, hundreds of nuclei were characterized, producing passage times of 0.5 ± 1.2 s for SW620 nuclei in type I constriction channel (n = 153), 0.045 ± 0.047 s for SW620 nuclei in type II constriction channel (n = 215) and 0.50 ± 0.86 s for A549 nuclei in type II constriction channel. In addition, neural network based pattern recognition was used to classify the nuclei isolated from SW620 and A549 cells, producing successful classification rates of 87.2% for diameters of nuclei, 85.5% for passage times of nuclei and 89.3% for both passage times and diameters of nuclei. These results indicate that the characterization of the mechanical properties of nuclei may contribute to the classification of different tumor cells.

KEYWORDS:

constriction channel; mechanical properties; microfluidics; nucleus isolation; single-nucleus analysis

PMID:
30063818
DOI:
10.1002/cyto.a.23386

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