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PLoS Negl Trop Dis. 2018 Jul 31;12(7):e0006693. doi: 10.1371/journal.pntd.0006693. eCollection 2018 Jul.

An attenuated replication-competent chikungunya virus with a fluorescently tagged envelope.

Author information

1
Blood Systems Research Institute, San Francisco, CA, United States of America.
2
University of California, San Francisco, San Francisco, CA, United States of America.
3
Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, TX, United States of America.
4
Department of Medical Biochemistry and Biophysics, Umeå University, Umeå, Sweden.

Abstract

BACKGROUND:

Chikungunya virus (CHIKV) is the most common alphavirus infecting humans worldwide, causing acute and chronically debilitating arthralgia at a great economic expense.

METHODOLOGY/PRINCIPAL FINDINGS:

To facilitate our study of CHIKV, we generated a mCherry tagged replication-competent chimeric virus, CHIKV 37997-mCherry. Single particle cryoEM demonstrated icosahedral organization of the chimeric virus and the display of mCherry proteins on virus surface. CHIKV 37997-mCherry is attenuated in both IFNαR knockout and wild-type mice. Strong anti-CHIKV and anti-mCherry antibody responses were induced in CHIKV 37997-mCherry infected mice.

CONCLUSIONS/SIGNIFICANCE:

Our work suggests that chimeric alphaviruses displaying foreign antigen can serve as vaccines against both aphaviruses and other pathogens and diseases.

PMID:
30063703
PMCID:
PMC6086482
DOI:
10.1371/journal.pntd.0006693
[Indexed for MEDLINE]
Free PMC Article

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