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Virology. 2018 Sep;522:271-280. doi: 10.1016/j.virol.2018.06.010. Epub 2018 Jul 26.

Coxsackievirus-B4E2 can infect monocytes and macrophages in vitro and in vivo.

Author information

1
Université de Lille, Faculté de Médecine, CHU de Lille, Laboratoire de Virologie/EA3610, F-59037 Lille, France.
2
Université de Lille, Faculté de Médecine, CHU de Lille, Laboratoire de Virologie/EA3610, F-59037 Lille, France; Université de Monastir, Faculté de Pharmacie, Laboratoire des Maladies Transmissibles et Substances Biologiquement Actives LR99ES27, Monastir, Tunisia; Université de Tunis El Manar, Faculté des Sciences de Tunis, Tunis, Tunisia.
3
Université de Picardie Jules Verne, CHU d'Amiens, Service d'Endocrinologie-Diabétologie-Nutrition, F-80054 Amiens, France.
4
Université de Monastir, Faculté de Pharmacie, Laboratoire des Maladies Transmissibles et Substances Biologiquement Actives LR99ES27, Monastir, Tunisia; Université de Tunis El Manar, Faculté des Sciences de Tunis, Tunis, Tunisia.
5
Université de Lille, Faculté de Médecine, CHU de Lille, Laboratoire de Virologie/EA3610, F-59037 Lille, France. Electronic address: didier.hober@chru-lille.fr.

Abstract

Viral RNA (vRNA) is found in mice inoculated with coxsackievirus-B4E2 (CV-B4E2). The CV-B4E2 infection of murine spleen cells in vitro is enhanced with CV-B4E2-infected mouse serum. It has been investigated whether monocyte/macrophages were targets of CV-B4E2 in mice. vRNA has been detected in spleen and bone marrow of infected animals. The levels of vRNA were higher in CD14+ cells than in CD14- spleen cells and in F4/80- cells than in F4/80+ spleen cells. Meanwhile, CD14+ cells and F4/80- cells were more permissive to CV-B4E2 in vitro and the infection was enhanced when the virus was mixed with immune serum. While CV-B4E2 infected BMDM cultures (98% F4/80+); however, the immune serum did not enhance the infection. In conclusion, CV-B4E2 infects monocytes (CD14+, F4/80-) and macrophages (CD14+, F4/80+) in vivo and immune serum can enhance the in vitro infection of these cells arising out of the spleen.

KEYWORDS:

Bone marrow; Enterovirus; Mouse; Serum; Spleen

PMID:
30056354
DOI:
10.1016/j.virol.2018.06.010
[Indexed for MEDLINE]
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