Format

Send to

Choose Destination
J Cell Sci. 2018 Aug 17;131(16). pii: jcs218297. doi: 10.1242/jcs.218297.

Phosphatidylinositol 4,5-bisphosphate regulates cilium transition zone maturation in Drosophila melanogaster.

Author information

1
Department of Molecular Genetics, University of Toronto, Toronto, Ontario, M5S 1A8, Canada.
2
Cell Biology Program, The Hospital for Sick Children, Toronto, Ontario, M5G 0A4, Canada.
3
Department of Molecular Genetics, University of Toronto, Toronto, Ontario, M5S 1A8, Canada julie.brill@sickkids.ca.
4
Institute of Medical Science, University of Toronto, Toronto, Ontario, M5S 1A8, Canada.

Abstract

Cilia are cellular antennae that are essential for human development and physiology. A large number of genetic disorders linked to cilium dysfunction are associated with proteins that localize to the ciliary transition zone (TZ), a structure at the base of cilia that regulates trafficking in and out of the cilium. Despite substantial effort to identify TZ proteins and their roles in cilium assembly and function, processes underlying maturation of TZs are not well understood. Here, we report a role for the membrane lipid phosphatidylinositol 4,5-bisphosphate (PIP2) in TZ maturation in the Drosophila melanogaster male germline. We show that reduction of cellular PIP2 levels through ectopic expression of a phosphoinositide phosphatase or mutation of the type I phosphatidylinositol phosphate kinase Skittles induces formation of longer than normal TZs. These hyperelongated TZs exhibit functional defects, including loss of plasma membrane tethering. We also report that the onion rings (onr) allele of DrosophilaExo84 decouples TZ hyperelongation from loss of cilium-plasma membrane tethering. Our results reveal a requirement for PIP2 in supporting ciliogenesis by promoting proper TZ maturation.

KEYWORDS:

Basal body; Cep290; Cilia; PtdIns(4,5)P2; Skittles; Transition zone

PMID:
30054387
DOI:
10.1242/jcs.218297
Free full text

Conflict of interest statement

Competing interestsThe authors declare no competing or financial interests.

Supplemental Content

Full text links

Icon for HighWire
Loading ...
Support Center