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Psychoneuroendocrinology. 2018 Nov;97:190-195. doi: 10.1016/j.psyneuen.2018.07.022. Epub 2018 Jul 19.

Radiolabel validation of cortisol in the hair of rhesus monkeys.

Author information

1
Wisconsin National Primate Research Center, University of Wisconsin, Madison, 1220 Capitol Court, Madison, WI, 53715, USA. Electronic address: akapoor@wisc.edu.
2
Wisconsin National Primate Research Center, University of Wisconsin, Madison, 1220 Capitol Court, Madison, WI, 53715, USA. Electronic address: darken@primate.wisc.edu.
3
Wisconsin National Primate Research Center, University of Wisconsin, Madison, 1220 Capitol Court, Madison, WI, 53715, USA. Electronic address: ziegler@primate.wisc.edu.

Abstract

Analysis of cortisol in hair has become a widespread tool for assessment of hypothalamic-pituitary-adrenal (HPA) axis activity because of its ease of collection and its ability to provide cumulative data over a period of months. In order to meaningfully interpret hair cortisol, however a direct validation by radio-metabolism is required to understand cortisol incorporation into hair. Tritiated [3H]-cortisol was IV administered to adult rhesus monkeys to determine 1) if [3H] is found in the hair after injection of [3H]-cortisol, 2) the growth rate of hair and 3) the form in which cortisol is found in hair. Samples of hair were collected from newly and previously shaved patches at 14-days and 28-days after [3H]-cortisol injection. Hair was processed by external wash, grinding, and hormone extractions. Samples were separated by high-performance liquid chromatography (HPLC) and fractions were collected and radioactivity assessed. We found [3H] incorporated into the hair by the 14-day hair collection and no new radioactivity was found by the 28-day collection. Individual hair growth rate was highly variable between monkeys, indicating that the between-subject hair growth patterns were not consistent. Importantly, for the first time, we showed that systemically administered [3H]-cortisol was found in the hair as [3H]-cortisol and [3H]-cortisone, as well as other glucocorticoid metabolites.

KEYWORDS:

Cortisol; HPLC-UV; Hair cortisol; Hair hormones; Radiolabel; Validation

PMID:
30053699
PMCID:
PMC6138524
[Available on 2019-11-01]
DOI:
10.1016/j.psyneuen.2018.07.022
[Indexed for MEDLINE]

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