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Plant Biotechnol J. 2019 Feb;17(2):499-504. doi: 10.1111/pbi.12993. Epub 2018 Oct 5.

Expanding the base editing scope in rice by using Cas9 variants.

Hua K1,2, Tao X1, Zhu JK1,3.

Author information

1
Shanghai Center for Plant Stress Biology, CAS Center of Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai, China.
2
University of Chinese Academy of Sciences, Beijing, China.
3
Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN, USA.

Abstract

Base editing is a novel genome editing strategy that enables irreversible base conversion at target loci without the need for double stranded break induction or homology-directed repair. Here, we developed new adenine and cytosine base editors with engineered SpCas9 and SaCas9 variants that substantially expand the targetable sites in the rice genome. These new base editors can edit endogenous genes in the rice genome with various efficiencies. Moreover, we show that adenine and cytosine base editing can be simultaneously executed in rice. The new base editors described here will be useful in rice functional genomics research and will advance precision molecular breeding in crops.

KEYWORDS:

Cas9 variants; adenine base editor; base editing; cytosine base editor; rice

PMID:
30051586
DOI:
10.1111/pbi.12993
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