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PLoS Genet. 2018 Jul 25;14(7):e1007511. doi: 10.1371/journal.pgen.1007511. eCollection 2018 Jul.

Velvet domain protein VosA represses the zinc cluster transcription factor SclB regulatory network for Aspergillus nidulans asexual development, oxidative stress response and secondary metabolism.

Author information

1
Department of Molecular Microbiology and Genetics and Goettingen Center for Molecular Biosciences (GZMB), University of Goettingen, Goettingen, Germany.
2
Molekulare Biotechnologie, Fachbereich Biowissenschaften, Goethe Universität Frankfurt, Frankfurt am Main, Germany.
3
Institute of Molecular Biology and Tumor Research (IMT), Philipps-University, Marburg, Germany.
4
Department of Biochemistry and Biophysics, Centre for Genome Research and Biocomputing, Oregon State University, Corvallis, OR, United States of America.
5
Institute of Biology, Molecular Microbiology and Biotechnology, Leiden University, Leiden, The Netherlands.

Abstract

The NF-κB-like velvet domain protein VosA (viability of spores) binds to more than 1,500 promoter sequences in the filamentous fungus Aspergillus nidulans. VosA inhibits premature induction of the developmental activator gene brlA, which promotes asexual spore formation in response to environmental cues as light. VosA represses a novel genetic network controlled by the sclB gene. SclB function is antagonistic to VosA, because it induces the expression of early activator genes of asexual differentiation as flbC and flbD as well as brlA. The SclB controlled network promotes asexual development and spore viability, but is independent of the fungal light control. SclB interactions with the RcoA transcriptional repressor subunit suggest additional inhibitory functions on transcription. SclB links asexual spore formation to the synthesis of secondary metabolites including emericellamides, austinol as well as dehydroaustinol and activates the oxidative stress response of the fungus. The fungal VosA-SclB regulatory system of transcription includes a VosA control of the sclB promoter, common and opposite VosA and SclB control functions of fungal development and several additional regulatory genes. The relationship between VosA and SclB illustrates the presence of a convoluted surveillance apparatus of transcriptional control, which is required for accurate fungal development and the linkage to the appropriate secondary metabolism.

PMID:
30044771
PMCID:
PMC6078315
DOI:
10.1371/journal.pgen.1007511
[Indexed for MEDLINE]
Free PMC Article

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