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J Proteome Res. 2018 Sep 7;17(9):2925-2936. doi: 10.1021/acs.jproteome.8b00022. Epub 2018 Aug 10.

Characterization of Naïve and Vitamin C-Treated Mouse Schwann Cell Line MSC80: Induction of the Antioxidative Thioredoxin Related Transmembrane Protein 1.

Author information

1
Leibniz-Institut für Analytische Wissenschaften -ISAS- e.V. , 44227 Dortmund , Germany.
2
Department of Neurology , University Hospital RWTH Aachen , Aachen , Germany.
3
Department of Neuropathology , Charité - Universitätsmedizin , Berlin , Germany.
4
JARA-BRAIN Institute Molecular Neuroscience and Neuroimaging , Forschungszentrum Jülich GmbH and RWTH Aachen University , Aachen , Germany.

Abstract

Schwann cells (SCs) are essential in the production of the axon-wrapping myelin sheath and provide trophic function and repair mechanisms in the peripheral nerves. Consequently, well-characterized SC in vitro models are needed to perform preclinical studies including the investigation of the complex biochemical adaptations occurring in the peripheral nervous system (PNS) under different (patho)physiological conditions. MSC80 cells represent a murine SC line used as an in vitro system for neuropathological studies. Here, we introduce the most abundant 9532 proteins identified via mass spectrometry-based protein analytics, and thus provide the most comprehensive SC protein catalogue published thus far. We cover proteins causative for inherited neuropathies and demonstrate that in addition to cytoplasmic, nuclear and mitochondrial proteins and others belonging to the protein processing machinery are very well covered. Moreover, we address the suitability of MSC80 to examine the molecular effect of a drug-treatment by analyzing the proteomic signature of Vitamin C-treated cells. Proteomic findings, immunocytochemistry, immunoblotting and functional experiments support the concept of a beneficial role of Vitamin C on oxidative stress and identified TMX1 as an oxidative stress protective factor, which might represent a promising avenue for therapeutic intervention of PNS-disorders with oxidative stress burden such as diabetic neuropathy.

KEYWORDS:

MSC80; Schwann cells; TMX1; diabetic neuropathy; protein cataloguing

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