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Methods Mol Biol. 2018;1830:141-148. doi: 10.1007/978-1-4939-8657-6_9.

Isolation of Arabidopsis Palisade and Spongy Mesophyll Cells.

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Division of Integrated Life Science, Graduate School of Biostudies, Kyoto University, Kyoto, Japan.
Division of Biological Science, Graduate School of Science and Technology, Nara Institute of Science and Technology, Nara, Japan.


Cell-type-specific transcription factors are key to deducing the distinct functions of specialized cells from gene expression profiles. Mesophyll is a major tissue for photosynthesis, and contributes about 80% of total RNA from leaves. Palisade and spongy mesophyll cells are sub-tissues that have different morphologies and physiologies. Thus, determining the palisade and spongy mesophyll-specific transcription factors from the respective sub-tissue-specific transcriptomes is vital to understanding or verifying functions of major plant tissues. One way in which gene expression profiles can be addressed is through direct isolation. Here, we present rapid and simple methods to isolate palisade and spongy mesophyll cells mechanically and enzymatically. This method provides a good yield of each isolated cell type, and the isolated cells can be used for various downstream applications.


Arabidopsis; Mesophyll; Palisade cells; Spongy cells; Tissue isolation

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