Format

Send to

Choose Destination
Heart Vessels. 2019 Jan;34(1):167-176. doi: 10.1007/s00380-018-1230-5. Epub 2018 Jul 24.

Sustained delivery of vascular endothelial growth factor using a dextran/poly(lactic-co-glycolic acid)-combined microsphere system for therapeutic neovascularization.

Author information

1
Department of Pathology, The First Affiliated Hospital, Nanchang University, 330006, Nanchang, People's Republic of China.
2
Department of Vascular Surgery, The Second Affiliated Hospital, Nanchang University, 330006, Nanchang, People's Republic of China.
3
Department of Vascular Surgery, The Second Affiliated Hospital, Nanchang University, 330006, Nanchang, People's Republic of China. cf970422@126.com.
4
Medical College, Nanchang University, 330006, Nanchang, People's Republic of China.

Abstract

We hypothesize that the controlled delivery of vascular endothelial growth factor (VEGF) using a novel protein sustained-release system based on the combination of protein-loaded dextran microparticles and PLGA microspheres could be useful to achieve mature vessel formation in a rat hind-limb ischemic model. VEGF-loaded dextran microparticles were fabricated and then encapsulated into poly(lactic-co-glycolic acid) (PLGA) microspheres to prepare VEGF-dextran-PLGA microspheres. The release behavior and bioactivity in promoting endothelial cell proliferation of VEGF from PLGA microspheres were monitored in vitro. VEGF-dextran-PLGA microsphere-loaded fibrin gel was injected into an ischemic rat model, and neovascularization at the ischemic site was evaluated. The release of VEGF from PLGA microspheres was in a sustained manner for more than 1 month in vitro with low level of initial burst release. The released VEGF enhanced the proliferation of endothelial cells in vitro, and significantly promoted the capillaries and smooth muscle α-actin positive vessels formation in vivo. The retained bioactivity of VEGF released from VEGF-dextran-PLGA microspheres potentiated the angiogenic efficacy of VEGF. This sustained-release system may be a promising vehicle for delivery of multiple angiogenic factors for therapeutic neovascularization.

KEYWORDS:

Ischemia; Microsphere; Neovascularization; Sustained release; Vascular endothelial growth factor

PMID:
30043157
DOI:
10.1007/s00380-018-1230-5
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Springer
Loading ...
Support Center