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Anal Biochem. 1985 Nov 1;150(2):264-72.

Separation of [1-3H]cellooligosaccharides by thin-layer chromatography: assay for cellulolytic enzymes.

Abstract

A thin-layer chromatographic method for the separation with high resolution of [1-3H]cellooligosaccharides on silica gel plates has been developed. Reducing end-labeled glucose through cellohexaose were separated on silica gel plates with three ascents of ethyl acetate:water: methanol (40:15:20; v:v) and each was extracted with an efficiency of 88 +/- 3%. Separations of cellooligosaccharides using other adsorbents, solvents, and impregnants are also described. This thin-layer chromatographic method facilitated analysis of the activity of cellulolytic enzymes.

PMID:
3004251
[Indexed for MEDLINE]
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