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Nano Lett. 2018 Aug 8;18(8):5294-5305. doi: 10.1021/acs.nanolett.8b02291. Epub 2018 Jul 26.

Nanoparticles Can Wrap Epithelial Cell Membranes and Relocate Them Across the Epithelial Cell Layer.

Author information

1
"Jožef Stefan Institute" , Jamova cesta 39 , SI-1000 Ljubljana , Slovenia.
2
Weatherall Institute of Molecular Medicine , University of Oxford , Headley Way , Oxford OX3 9DS , United Kingdom.
3
Jožef Stefan International Postgraduate School , Jamova cesta 39 , SI-1000 Ljubljana , Slovenia.
4
Center of Excellence NAMASTE , Jamova cesta 39 , SI-1000 Ljubljana , Slovenia.
5
Systems Biology Ireland , University College Dublin , Dublin 4 , Ireland.
6
School of Medicine and Medical Science , University College Dublin , Dublin 4 , Ireland.
7
Faculty of Mathematics and Physics , University of Ljubljana , Jadranska 19 , SI-1000 Ljubljana , Slovenia.
8
Institute of Applied Optics , Friedrich-Schiller University , Jena 07749 , Germany.
9
Leibniz Institute of Photonic Technology (IPHT) , Jena 07745 , Germany.

Abstract

Although the link between the inhalation of nanoparticles and cardiovascular disease is well established, the causal pathway between nanoparticle exposure and increased activity of blood coagulation factors remains unexplained. To initiate coagulation tissue factor bearing epithelial cell membranes should be exposed to blood, on the other side of the less than a micrometre thin air-blood barrier. For the inhaled nanoparticles to promote coagulation, they need to bind lung epithelial-cell membrane parts and relocate them into the blood. To assess this hypothesis, we use advanced microscopy and spectroscopy techniques to show that the nanoparticles wrap themselves with epithelial-cell membranes, leading to the membrane's disruption. The membrane-wrapped nanoparticles are then observed to freely diffuse across the damaged epithelial cell layer relocating epithelial cell membrane parts over the epithelial layer. Proteomic analysis of the protein content in the nanoparticles wraps/corona finally reveals the presence of the coagulation-initiating factors, supporting the proposed causal link between the inhalation of nanoparticles and cardiovascular disease.

KEYWORDS:

STED microscopy; TiO2 nanoparticles; coagulation cascade interference; lipid wrapping; membrane disruption; tissue factor relocation

PMID:
30039976
PMCID:
PMC6089500
DOI:
10.1021/acs.nanolett.8b02291
[Indexed for MEDLINE]
Free PMC Article

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