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Mol Cell Biol. 2018 Sep 28;38(20). pii: e00259-18. doi: 10.1128/MCB.00259-18. Print 2018 Oct 15.

Role of Circular RNA DLEU2 in Human Acute Myeloid Leukemia.

Wu DM1,2, Wen X1,2, Han XR1,2, Wang S1,2, Wang YJ1,2, Shen M1,2, Fan SH1,2, Zhang ZF1,2, Shan Q1,2, Li MQ1,2, Hu B1,2, Chen GQ3, Lu J4,2, Zheng YL4,2.

Author information

1
Key Laboratory for Biotechnology on Medicinal Plants of Jiangsu Province, School of Life Science, Jiangsu Normal University, Xuzhou, People's Republic of China.
2
College of Health Sciences, Jiangsu Normal University, Xuzhou, Jiangsu Province, People's Republic of China.
3
State Key Laboratory of Pharmaceutical Biotechnology, MOE Key Laboratory of Model Animal for Disease Study, Model Animal Research Center, Nanjing University, Nanjing, People's Republic of China.
4
Key Laboratory for Biotechnology on Medicinal Plants of Jiangsu Province, School of Life Science, Jiangsu Normal University, Xuzhou, People's Republic of China lu-jun75@163.com ylzheng@jsnu.edu.cn.

Abstract

In the current study, we were interested in exploring the molecular mechanism of circular RNA DLEU2 (circRNA-DLEU2) (hsa_circ_0000488) and microRNA 496 (miR-496), as well as PRKACB, in human acute myeloid leukemia (AML) cell activities. The RNA expression levels of circRNA-DLEU2, hsa-miR-496, and PRKACB were assessed by quantitative real-time PCR (qRT-PCR). The proliferation and apoptosis abilities of the cells were determined by CCK8 assay and flow cytometry analysis. Target relationships between circRNA-DLEU2 and miR-496, as well as PRKACB, were analyzed by luciferase reporter assay and probe assay. Immunoblotting assays were used to detect the protein expression level of PRKACB. We also did in vivo experiments to observe tumor formation after overexpression of circRNA-DLEU2. Our data showed that circRNA-DLEU2 was upregulated in AML tissues and cells, which promoted AML cell proliferation and inhibited cell apoptosis. circRNA-DLEU2 promoted AML tumor formation in vivo miR-496 was inhibited by circRNA-DLEU2 and was downregulated in AML tissues. circRNA-DLEU2 inhibited miR-496 expression and promoted PRKACB expression. miR-496 antagonized the effects of PRKACB on MOLM-13 cell proliferation and apoptosis. Collectively, circRNA-DLEU2 accelerated human AML by suppressing miR-496 and promoting PRKACB expression.

KEYWORDS:

AML; PRKACB; circRNA-DLEU2; hsa-miR-496

PMID:
30037980
PMCID:
PMC6168983
DOI:
10.1128/MCB.00259-18
[Indexed for MEDLINE]
Free PMC Article

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