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Anal Chem. 2018 Aug 21;90(16):9813-9820. doi: 10.1021/acs.analchem.8b01759. Epub 2018 Aug 1.

Particle-Templated Emulsification for Microfluidics-Free Digital Biology.

Author information

1
Department of Bioengineering and Therapeutic Sciences, California Institute for Quantitative Biosciences , University of California , San Francisco , California 94158 , United States.
2
Chan Zuckerberg Biohub , San Francisco , California 94158 , United States.

Abstract

The compartmentalization of reactions in monodispersed droplets is valuable for applications across biology. However, the requirement of microfluidics to partition the sample into monodispersed droplets is a significant barrier that impedes implementation. Here, we introduce particle-templated emulsification, a method to encapsulate samples in monodispersed emulsions without microfluidics. By vortexing a mixture of hydrogel particles and sample solution, we encapsulate the sample in monodispersed emulsions that are useful for most droplet applications. We illustrate the method with ddPCR and single cell culture. The ability to encapsulate samples in monodispersed droplets without microfluidics should facilitate the implementation of compartmentalized reactions in biology.

PMID:
30033717
PMCID:
PMC6122844
DOI:
10.1021/acs.analchem.8b01759
[Indexed for MEDLINE]
Free PMC Article

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