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Cell. 2018 Jul 26;174(3):730-743.e22. doi: 10.1016/j.cell.2018.06.019. Epub 2018 Jul 19.

A Complete Electron Microscopy Volume of the Brain of Adult Drosophila melanogaster.

Author information

1
Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA.
2
Coleman Technologies, Newtown Square, PA 19073, USA.
3
Hudson Price Designs, Hingham, MA 02043, USA.
4
Division of Neurobiology, MRC Laboratory of Molecular Biology, Cambridge CB2 0QH, UK; Department of Zoology, University of Cambridge, Cambridge CB2 3EJ, UK.
5
Department of Computer Science, Johns Hopkins University, Baltimore, MD 21218, USA.
6
Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA. Electronic address: bockd@janelia.hhmi.org.

Abstract

Drosophila melanogaster has a rich repertoire of innate and learned behaviors. Its 100,000-neuron brain is a large but tractable target for comprehensive neural circuit mapping. Only electron microscopy (EM) enables complete, unbiased mapping of synaptic connectivity; however, the fly brain is too large for conventional EM. We developed a custom high-throughput EM platform and imaged the entire brain of an adult female fly at synaptic resolution. To validate the dataset, we traced brain-spanning circuitry involving the mushroom body (MB), which has been extensively studied for its role in learning. All inputs to Kenyon cells (KCs), the intrinsic neurons of the MB, were mapped, revealing a previously unknown cell type, postsynaptic partners of KC dendrites, and unexpected clustering of olfactory projection neurons. These reconstructions show that this freely available EM volume supports mapping of brain-spanning circuits, which will significantly accelerate Drosophila neuroscience. VIDEO ABSTRACT.

KEYWORDS:

Drosophila melanogaster; connectomics; electron microscopy; image stitching; mushroom body; neural circuits; olfaction

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PMID:
30033368
PMCID:
PMC6063995
DOI:
10.1016/j.cell.2018.06.019
[Indexed for MEDLINE]
Free PMC Article

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