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Blood. 2018 Sep 13;132(11):1198-1207. doi: 10.1182/blood-2018-05-851360. Epub 2018 Jul 19.

RH genotype matching for transfusion support in sickle cell disease.

Author information

1
Department of Pediatrics and.
2
Department of Biomedical and Health Informatics, The Children's Hospital of Philadelphia, Philadelphia, PA.
3
Laboratory of Immunohematology and Genomics, New York Blood Center, New York, NY.
4
Department of Pathology and Laboratory Medicine, The Children's Hospital of Philadelphia, Philadelphia, PA; and.
5
National Molecular Laboratory at the American Red Cross, Philadelphia, PA.

Abstract

Rh alloimmunization remains a challenge for patients with sickle cell disease (SCD) despite transfusion of serologic Rh C, E, and K antigen-matched red cells. Inheritance of altered RH alleles contributes to the prevalence of Rh antibodies after blood transfusion in patients with SCD and explains approximately one-third of cases. The remainder seem to be stimulated by altered Rh proteins on African American donor red cells. Matching patients with donors on the basis of RH genotype may mitigate Rh alloimmunization, but the feasibility and resources required are not known. We compared RH allele frequencies between patients with SCD (n = 857) and African American donors (n = 587) and showed that RH allele frequencies are similar. Overall, 29% of RHD and 53% of RHCE alleles are altered in patients and African American donors. We modeled RH genotype matching compared with serologic Rh D, C, and E, along with K antigen matching, and found that approximately twice the number of African American donors would be required for RH genotype vs Rh serologic matching at our institution. We demonstrated that African American donor recruitment is necessary to maintain an adequate supply of C-, E-, and K-negative donor units to avoid depleting the Rh-negative (RhD-) blood supply. Our results suggest that prophylactic RH genetic matching for patients with SCD is feasible with a donor pool comprised primarily of African-Americans and would optimize the use of our existing minority donor inventory. The current cost of RH genotyping all minority donors and management of the data remain limiting factors.

PMID:
30026182
DOI:
10.1182/blood-2018-05-851360
[Indexed for MEDLINE]

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