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Food Chem Toxicol. 2018 Oct;120:305-320. doi: 10.1016/j.fct.2018.07.028. Epub 2018 Jul 17.

Antioxidant, anti-inflammatory and synergistic anti-hyperglycemic effects of Malaysian propolis and metformin in streptozotocin-induced diabetic rats.

Author information

1
Department of Physiology, School of Medical Sciences, Universiti Sains Malaysia, 16150, Kelantan, Malaysia; Department of Physiology, Faculty of Basic Medical Sciences, College of Medical Sciences, University of Calabar, P.M.B. 1115, Calabar, Cross River State, Nigeria.
2
Department of Physiology, School of Medical Sciences, Universiti Sains Malaysia, 16150, Kelantan, Malaysia.
3
Department of Physiology, School of Medical Sciences, Universiti Sains Malaysia, 16150, Kelantan, Malaysia; Unit of Integrative Medicine, School of Medical Sciences, Universiti Sains Malaysia, 16150, Kelantan, Malaysia. Electronic address: mahaneem@usm.my.

Abstract

Diabetes mellitus is characterized by hyperglycemia which causes oxidative stress. Propolis has been reported to have antihyperglycemic and antioxidant potentials. The present study therefore examined the anti-hyperglycemic, antioxidant and anti-inflammatory activities of Malaysian propolis (MP) using streptozotocin-induced diabetic rats. Ethanol extract of MP showed in vitro antioxidant (DPPH, FRAP and H2O2 radical scavenging) and α-glucosidase inhibition activities. Male Sprague Dawley rats were either treated with distilled water (normal control and diabetic control), MP (300 mg/kg b. w.), metformin (Met) (300 mg/kg b. w.) or both. After four weeks, fasting blood glucose decreased, while body weight change and serum insulin level increased significantly in MP, Met and MP + Met treated diabetic groups compared to diabetic control (DC) group. Furthermore, pancreatic antioxidant enzymes, total antioxidant capacity, interleukin (IL)-10 and proliferating cell nuclear antigen increased, while malondialdehyde, nuclear factor-kappa B (p65), tumor necrosis factor alpha, IL-1β and cleaved caspase-3 decreased significantly in the treated diabetic groups compared to DC group. Histopathology of the pancreas showed increased islet area and number of beta cells in the treated groups, compared to DC group, with D + MP + Met group comparable to normal control. We conclude that MP has anti-hyperglycemic, antioxidant, anti-inflammatory and antiapoptotic potentials, and exhibits synergistic effect with metformin.

KEYWORDS:

Antioxidant; Diabetes; Inflammation; Malaysian propolis; Metformin

PMID:
30026088
DOI:
10.1016/j.fct.2018.07.028
[Indexed for MEDLINE]

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